6 What is the molecular weight of the large subunit of RuB A 14000 Daltons B 55000 Daltons C 37 kDa D 50 kDa 7 Which type of

Question

6 What is the molecular weight of the large subunit of RuB A 14000 Daltons B 55000 Daltons C 37 kDa D 50 kDa 7 Which type of buffer is used during the elution step in ion exchange chromatography to displace proteins from the beads A High pH buffer B Low salt buffer C High salt buffer D Organic solvent 8 What is the purpose of heating samples with sample buffer before protein electrophoresis A To precipitate the proteins B To renature the proteins C To denature the proteins and reduce disulfide bonds D To sterilize the samples True False Questions 1 RuBisCO is an efficient enzyme with high CO2 fixation rates True False Secondary structure of proteins involves hydrogen bonding between polypeptide chains True False 2 In ion exchange chromatography the stationary phase is typically nonpolar True False 3 Proteins can be separated based on their solubility differences using ammonium sulfate precipitation True False Calculation Questions 1 If you have a protein solution of 100 mL how much ammonium sulfate in gram is needed to reach a saturation point of 37 given that the required amount is 210 g L 2 For a 50 saturation of ammonium sulfate how many grams would be neede per liter

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