a If positive control is homozygous recessive would you be able to determine if restriction digestion was effective b Forward

Question

a If positive control is homozygous recessive would you be able to determine if restriction digestion was effective b Forward and reverse primers complementary would anneal rather than anneal to the DNA target c What are the major components of PCR Dilution questions serial dilutions percent solutions w v v v molarity a Prepare 1L DMEM 10 FBS 1 Glutamine 1 Pen strep b Prepare 200mL of 0 1 M CaCl from stock of 10M CaCl C

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