Aprotein sample complex consists of two proteins a smaller protein X and a larger protein Y Protein X is composed of tw

Question

Aprotein sample complex consists of two proteins a smaller protein X and a larger protein Y Protein X is composed of tw polypeptide chains linked by disulfide bonds Protein Y is composed of three polypeptide chains linked by disulfide bonds 3 The complex is analyzed by native PAGE reducing SDS PAGE and non reducing SDS PAGE Native PAGE does not include sodium dodecyl sulfate or SDS Reducing SDS PAGE uses both SDS and a reducing agent in the buffer Non reducing SDS PAGE uses SDS but no reducing agent Match each gel pattern to an experimental method The first lane in each gel is a molecular weight ladder Native PAGE 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa 1000 kDa 500 kDa 250 kDa 100 kDa 50 kDa 30 kDa Non reducing SDS PAGE Answer Bank 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa What are the molecular weights of the protein X polypeptides Reducing SDS PAGE

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