Which of the following must be considered when estimating TMRCA (time of most recent common ancestor) for a Species?(I discussed

Question

Which of the following must be considered when estimating TMRCA (time of most recent common ancestor) for a Species?(I discussed this at length in the Notes of Ch 9.)
The rates of mutation in the sections of DNA being analyzed for the purpose of finding TMRCA.
The average/effective generation time for the species (as a factor in the molecular clock)
Population size for the species (as a factor in the molecular clock)
Whether or not the sections of DNA being analyzed for TMRCA are under selection or not.

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