Question:

You are a researcher investigating the role of the abc gene in tumor formation. You suspect that the abc is expressed in lung cells based on previous research, but the hypothesis has yet to be tested. Even though abc is a known regulator of transcription,

Last updated: 7/26/2022

You are a researcher investigating the role of the abc gene in tumor formation. You suspect that the abc is expressed in lung cells based on previous research, but the hypothesis has yet to be tested. Even though abc is a known regulator of transcription,

You are a researcher investigating the role of the abc gene in tumor formation. You suspect that the abc is expressed in lung cells based on previous research, but the hypothesis has yet to be tested. Even though abc is a known regulator of transcription, you are uncertain what sequence/gene it regulates. Which of the following methods could be used to answer these questions? (i) Create a DNA microarray to see if abc is activated in lung cells. (ii) Make a lung cell lysate and determine the amount of ABC using western blot and use ChIP to select for the DNA sequences that ABC binds. (iii) Use Northern blot to determine the amount of abc RNA present and then western blot to determine the amount of the ABC protein present in the lung cells. (iv) Create labelled lung cell DNA fragments that are potential binding sites of ABC and perform EMSA to determine if it binds.