3. A researcher has identified a new protein, protein A,

Last updated: 7/24/2022

3. A researcher has identified a new protein, protein A, which they hypothesise interacts with protein B. To investigate this interaction, the researcher first wants to determine if the two proteins colocalise in the cell. List the type of microscope that would be best to study this, and indicate what other manipulations or preparations the researcher would need to do to prepare the sample for visualisation. (3 marks) To know the protein-protein interaction, we have to label the proteins with fluorescent dyes. We need to excite these dyes with suitable wavelength and emitted light will be absorbed and show the protein-protein interaction. The best microscope to study the protein-protein interaction is fluorescent microscope, and fluorescent resonance energy transfer (FRET) is a popular technique used for the study of protein-protein interaction. Using your answer from the previous question, give one pro and one con for using that sample preparation approach. (2 marks)