A biochemistry laboratory student used the Bradford protein

Last updated: 4/24/2023

A biochemistry laboratory student used the Bradford protein assay to measure the lysozyme protein content in egg whites The Bradford protein assay is a spectrophotometric technique that uses a dye Coomassie Brilliant Blue whose absorption undergoes a spectroscopic shift when bound to a protein In the unbound state Coomassie Brilliant Blue displays a red color As protein binds to the dye its absorbance at 595 nm increases and the dye changes to a blue color The student prepared a 3 600 mL sample containing 2 mg mL Coomassie Brilliant Blue To this sample 300 0 L of 70 0 g mL lysozyme was added and an absorbance of 0 264 was measured at 595 nm Calculate the corrected absorbance for this sample atod absorbance