Chemical synthesis of DNA Linking first nucleotide to column

Last updated: 10/4/2023

Chemical synthesis of DNA Linking first nucleotide to column Removing oligonucleotide from column Purifying oligonucleotide Washing Detritylation Washing Activation and coupling 100 Washing Capping Oxidation n cycles What are the special nucleotide that used in this synthesis How is it different from regular nucleotide The direction of chemical synthesis What are on the platform column What would be the first nucleotide that linked to the platform column 5 GGATCGGAAT 3 How many bases are on the oligonucleotides after n cycles What technique is used to purify oligonucleotides Explain the following steps Detritylation Activation and Coupling Capping and Oxidation including washing after