Counting cells: You have 8 ml of a cell suspension. You mix

Last updated: 8/4/2022

Counting cells: You have 8 ml of a cell suspension. You mix together 0.25 ml of 0.4% (w/v) Trypan Blue with 0.25 ml of HBSS buffer and 0.25 ml of your cell suspension. When you count this in a haemocytometer your average count per square (of a volume of 0.1 μl each) is 25 cells. How many cells per ml do you have in the original suspension? a. 500000 b. 250000 c. 6000000 d. 1000000 e. 750000