Draw a diagram that illustrates the experimental design

Last updated: 12/13/2023

Draw a diagram that illustrates the experimental design described in this excerpt Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells To identify candidate reprogramming factors we compiled a list of genes with enriched expression in human ES cells relative to that of other cell types and prioritized the list based on known involvement in the establishment or maintenance of pluripotency We then cloned these genes into a lentiviral vector to screen for combinations of genes that could reprogram the differentiated derivatives of an OCT4 knock in human ES cell line generated through homologous recombination In this cell line the expression of neomycin phosphotransferase which makes cells resistant to geneticin is driven by an endogenous OCT4 promoter a gene that is highly expressed in pluripotent cells but not in differentiated cells Thus reprogramming events reactivating the OCT4 promoter