How many nucleotide s should you add to your forward primer to adjust the reading frame if you are planning to use the

Question

How many nucleotide s should you add to your forward primer to adjust the reading frame if you are planning to use the restriction site BamH I knowing that the subcloning will be done in pGEX5X 3 pGEX 5X 3 27 4586 01 Factor Xa lle Glu Gly Arg Gly lle Pro Arg Asn Ser Arg Val Asp Ser Ser Gly Arg lle Val Thr Asp ATC GAA GGT CGT GGG ATC CCC AGG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA BamHI EcoRI Sal I Smal Xho I Not I Stop codons 2 1

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