Question:

Imagine that you repeat the Benzer tRNA Selection experiment

Last updated: 5/13/2023

Imagine that you repeat the Benzer tRNA Selection experiment

Imagine that you repeat the Benzer tRNA Selection experiment with modifications as follows 1 Synthesize mRNA containing U s and C s only poly UC in random order 2 Convert the amino acid Phenylalanine Phe on its tRNA to the amino acid Tyrosine Tyr as shown below 3 Mix your poly UC RNA your artificial tRNA and cell extract contains ribosomes amino acids all normal tRNAS and the energy source for translation Phe 5 Tyr 5 GAA GAA Phenylalanine Phe is encoded by UUU and UUC while Tyrosine Tyr is encoded only by UAC and UAU Given this and given what you know about tRNA selection will Tyrosine be found in the resulting polypeptides Assume translation will happen even without a start codon No because the ribosome selects the amino acid regardless of the anticodon sequence Yes because all of the normal tRNAs including tRNATyr are present in the assay Yes because the amino acid is selected based on the tRNA anticodon sequence None of the above