lon exchange column We will be using Q Sepharose fast flow

Last updated: 9/21/2023

lon exchange column We will be using Q Sepharose fast flow columns to perform ion exchange chromatography to further purify RuBisCo from other proteins The resin in the column consists of tiny spherical beads of cross linked 6 agarose with bound dextran having a positive charge known as an anionic exchanger thus allowing negatively charged proteins to adhere to the beads in the column Applying our samples through the columns will enable us to separate proteins based on their affinities for the positively charged column resin There is approximately 5 mL of Q Sepharose packed into the column