Spleen cells are taken from a mouse immunized with the LCM

Last updated: 6/23/2023

Spleen cells are taken from a mouse immunized with the LCM virus 2 assays are used to determine antigen specific functional activity of the s Assay 1 the spleen cells T cells are incubated with macrophages that have been briefly exposed to LCM virus phagocytized the virus produs response Assay 2 the spleen cells are incubated with LCM infected target cells lysis of the infected target cells is considered a positive response Activity of which cell population is detected in Assay 2