The activity of chymotrypsin using N-acetyl-l-phenylalanine p-nitrophenyl ester as a substrate at pH = 7.0 was monitored

Question

The activity of chymotrypsin using N-acetyl-l-phenylalanine p-nitrophenyl ester as a substrate at pH = 7.0 was monitored spectroscopically in a 1 cm cuvette. What is the concentration of product produced if an absorbance of 0.6 is obtained at 415 nm, given a molar absorptivity of 15,000 L mol¹ cm¹?
There is not enough information to calculate the concentration.
40 uM
2.5 nM
9 mM

Kunduz · Accepted Answer

Click to see the answer