When analyzing lipase activity in the lab why did no fat digestion occur in the tube with 3 ml litmus cream 3 ml lipase bile

Question

When analyzing lipase activity in the lab why did no fat digestion occur in the tube with 3 ml litmus cream 3 ml lipase bile salts 0 degrees Celsius O The temperature was too high The temperature was too low The enzyme and substrate were at the correct temperature There was no enzyme present

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