When analyzing lipase activity in the lab why did no fat digestion occur in the tube with 3ml H 20 3 ml litmus cream 37 degrees

Question

When analyzing lipase activity in the lab why did no fat digestion occur in the tube with 3ml H 20 3 ml litmus cream 37 degrees Celsius There was no substrate There was no bile present There was no enzyme present The temperature was too low 4

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