Question:
You are to target 3 different proteins, using 3 different
Last updated: 7/8/2022
You are to target 3 different proteins, using 3 different techniques (one technique per protein). Use any of the techniques listed below to study the given pathway. You need to explain which technique you are using and which protein you are targeting and explain why (for both the technique and the protein choice). Finally, explain how your technique will allow you to study the pathway; in other words what will happen when the pathway is disrupted? In other words, how will you know the role of your target protein? Technique choices: Dominant-negative mutant Temperature-sensitive mutant Antibodies Non-degradable forms of a protein non-hydrolyzable ATP