Biology Questions

uestion 8 Points 2 ennedy and Nixon debate before the 1960 presidential election Based on your own nowledge why did television viewers think Kennedy won the debate O He was more knowledgeable than expected O He landed more jabs at Nixon O He looked and sounded better on TV He had more political experience Reset Reset E this ass Questio 1 5 Res 9

Alan Shepard John Glenn Based on your own knowledge these three men were the first to O break the sound barrier in a jet O fully orbit the earth O travel in space Oland on the moon

Question 7 Points 1 Read this quotation the key which can unlock the door to the Great Society Based on your own knowledge which of President Johnson s initiatives does this quotation refer O Civil rights O Education Health O Housing Complete Later Complete Rese Reset this as Questi 5 9 Re

Question 3 Points 2 Why did tension between Cuba and the US grow O Cuba did not let prisoners go after the Bay of the Pigs attack O Russians built missile bases in Cuba O Cuba performed missile testing near the Florida Keys O The US deported a lot of Cubans

Look at this photo This is a still photograph from a political campaign commercial in the 1964 election This little girl s picture dissolved into a mushroom cloud Based on your own knowledge who ran the ad O Lyndon Johnson Barry Goldwater The Republican National Committee O The Democratic National Committee Reset Reset buta this assess Reset Questions 1 5 9 10

Question 2 Points 2 How would you view both the Bay of Pigs and the Cuban Missile Crisis if you were a supporter of Fidel Castro Two important setbacks O A victory in the Bay of Pigs and a loss in the Cuban Missile Crisis O Two important triumphs He won at the Bay of Pigs while the Soviets lost face in the Cuban Missile Crisis not Castro Anxious about his future as dictator Que R th 5 9 1

What kind of character does the narrator attempt to depict in the excerpt given Hester has the strength and courage to live her life as she determines it Hester has the power to attract Opeople s attention and attain what she wanted in life Hester feels guilty for her adultery and gets into her shell Hester wants to kill her O daughter and commit suicide so

DUD Trusting no man as his friend he could not recognize his enemy when the latter actually appeared Which of the following options best explains Dimmesdale s behavior 000 OOO OOO Mr Dimmesdale is an introvert O and never discloses his secrets to anybody Q Caight C poor that he could not recognize people near him Mr Mr Dimmesdale does not want O his secret love affair to come to light Dimmesdale trusts

4 3 U2 In aquatic ecosystems carbon is present as dissolved carbon dioxide and hydrogen carbonate ions Outline the process that converts CO to hydrogen carbonate ion in water leading to a reduction of the pH in the water 4 3 U10 Animals such as reef building corals and Mollusca have hard parts that are composed of calcium carbonate and can become fossilized in limestone State that hard shells such as in mollusk and coral are made of calcium carbonate 4 3 S1 Construct a diagram of the carbon cycle Define pool and flux Draw a diagram of the terrestrial carbon cycle Paste here Draw a diagram of the aquatic carbon cycle Paste here Option C Draw a diagram of the Nitrogen cycle Paste here Draw a diagram of the Phosphorus cycle Paste here I

4 1 U7 A community is formed by populations of different species living together and interacting with each other Define species population and community Give an example of a community of organisms 4 1 U8 A community forms an ecosystem by its interactions with the abiotic environment Define abiotic and ecosystem 9 4 U3 Most flowering plants use mutualistic relationships with pollinators in sexual reproduction Define mutualism Explain an example of mutualism between a flowering plant and its pollinator

4 1 011 Ecosystems have the potential to be sustainable over long periods of time Define sustainability Give an example of an unsustainable practice Outline three requirements of a sustainable ecosystem

DNA Extraction of Your Samples for PCR 1 Prepare three samples Your food sample the GMO positive sample and the GMO negative sample Reducing the chance of contamination is critical Wipe all tools used micropestle and razor blade with alcohol wipes and do not switch tools between samples Crush or dice up a small amount of your sample with a micropestle or razor blade Add the crushed sample to a clean 1 5 mL Eppendorf tube to a level about halfway to the 0 1 mL mark Label the tube with the sample type and your group color Repeat the process for the GMO negative and GMO positive sample 2 Add 100 L of Edward s buffer to each tube containing the plant or food material Remember to change tips between samples to decrease the chance of contamination 3 Twist a clean micropestle against the inner surface of the 1 5 mL Eppendorf tube to forcefully grind the plant tissue or food product for 1 min 4 Add an additional 900 L of Edward s buffer to each tube containing the ground sample Grind briefly to remove tissue from the micropestle Vortex the tubes for 5 sec 5 6 Boil the samples for 5 min in a water bath using a circular floater Wrap tube openings with parafilm or use lid locks to keep lids from popping open during heating 7 Place the tubes in a balanced microcentrifuge and spin for 2 min at 12 000 rpms to pellet cell and food debris 8 Transfer 350 L of each supernatant to a fresh tube Maintain labels for each sample type and group color Be careful not to disturb the pelleted debris when transferring the supernatant Discard old tubes containing the precipitates 9 Add 400 L of isopropanol to each tube of supernatant 10 Mix by inverting the tubes several times and leave at room temperature for 3 min 11 Place the tubes in a balanced microcentrifuge and spin for 5 min at 14 000 rpms Align tubes in the rotor with the cap hinges pointing outward Nucleic acids will collect on the tube side under the hinge and pellet out of solution during centrifugation 12 Carefully pour off the supernatant from each tube and then completely remove the remaining liquid with a micropipette set at 100 uL

13 Air dry the pellets for 10 min to evaporate remaining isopropanol 14 Add 400 L of ethanol to each tube the air dried pellets 15 Mix by inverting the tubes several times and leave at room temperature for 3 min 16 Place the tubes in a balanced microcentrifuge and spin for 5 min at 14 000 rpms Align tubes in the rotor with the cap hinges pointing outward Nucleic acids will collect on the tube side under the hinge during centrifugation 17 Carefully pour off the supernatant from each tube and then completely remove the remaining liquid with a micropipette set at 100 L 18 Air dry the pellets for 10 min to evaporate remaining ethanol 19 Add 100 L of TE RNase A buffer to each tube Dissolve the nucleic acid pellet by pipetting in and out Take care to wash down the side of the tube underneath the hinge where the pellet formed during centrifugation 20 Incubate TE RNAse A buffer and DNA mixture at room temperature for 5 min 21 Microcentrifuge the tubes for 1 min at 12 000 rpms to pellet any material that did not go into the solution 22 DNA may be used immediately or stored at 20 C until you are ready to continue with the amplification of the DNA Keep the DNA on ice as you proceed Edward s Buffer dH O 1 M Tris pH 8 0 5 M NaCl 0 5 M EDTA 10 SDS TE RNAse A Buffer dH O 0 01 M Tris pH 8 0 2 mM EDTA RNase A dissolved in glacial acetic

1 What is the difference between electrical and chemical signals 2 What is the target cell a What is required for any target to receive any signal b What does specificity affinity competition and saturation affect the response 3 What is the difference between local communication and long distance communication a Name at least 3 examples of local communication b What are the methods cells use for long distance communication 4 What is the difference between autocrine and paracrine signals

Which Career Pathway is right for you THE RIASEC TEST Follow these easy steps to see where your interests are 1 Read each statement If you agree with the statement fill in the circle There are no wrong answers 1 I like to work on cars 2 I like to do puzzles 3 I am good at working independently I like to work in teams 4 5 6 I like to organize things files desks offices 7 8 I am an ambitious person I set goals for myself 9 I like to build things I like to read about art and music I like to have clear instructions to follow 10 I like to try to influence or persuade people 11 I like to do experiments 12 I like to teach or train people 13 I like trying to help people solve their problems I like to take care of animals 14 15 I wouldn t mind working 8 hours per day in an office 16 I like selling things 17 I enjoy creative writing 18 I enjoy science 19 I am quick to take on new responsibilities 20 I am interested in healing people 21 I enjoy trying to figure out how things work O Total Total O O O OO O 2 Add up the number of filled in circles in each column and then add the two columns together for a grand total Using your grand total scores from above transfer the scores for each letter into the appropriate column below R Realistic I Investigative A Artistic Total S Social Total E Enterprising Total C Conventional Total O R Realistic These people are often good at mechanical or athletic jobs Good college majors for Realistic people are Agriculture O O O O Which Career Pathway is right for you RESULTS OF THE RIASEC TEST I like putting things together or assembling things I am a creative person 24 I pay attention to details 25 I like to do filing or typing 22 23 26 I like to analyze things problems situations 27 I like to play instruments or sing I enjoy learning about other cultures 28 29 I would like to start my own business Total 30 I like to cook 31 I like acting in plays 32 33 34 35 36 37 38 I am a practical person I like working with numbers or charts I like to get into discussions about issues I am good at keeping records of my work I like to lead I like working outdoors I would like to work in an office 39 I m good at math 40 I like helping people 41 I like to draw 42 I like to give speeches R A Grand Total Total Take the three letters with the highest scores and record them under My Interest Code MY INTEREST CODE O O C C O C S Social These people like to work with other people rather than things Good college majors for Social people are O O O O EIC 5 Turn the page to see what this means

56 What is resting membrane potential a Definition b C Does the ICF have an overall positive or negative charge Does the ECF have an overall positive or negative charge d Which ion is mainly responsible for setting the RMP and why 57 What is depolarization repolarization and hyperpolarization Definition a b What is the overall effect of the membrane mV normally for each c Which ions are involved and are they entering or exiting the cell

Photosynthesis is the process plants use to convert sunlight into chemical energy The process of photosynthesis can be modeled to illustrate the most important aspects of the natural phenomenon Through this process plants use energy in sunlight to turn carbon dioxide gas and water into sugar Plants then use this sugar to grow At the same time plants produce oxygen gas as a waste product which animals breathe Read the prompt and evaluate the diagram above Formulate a cause and effect prediction that can be used to test the assumptions in the model 1x A B C limiting water will limit sugar production of the plant limiting sunlight will limit sugar production of the plant limiting carbon dioxide will limit sugar production of the plant D limiting sunlight water or carbon dioxide will limit sugar production of the plant 2

Question 7 Points 1 The first president to benefit from instant popularity by appearing on TV to promote himself in commercials was Adlai Stevenson O Dwight Eisenhower O Storm Thurmond O Harry Truman

Which of the following issues were Dixiecrats for The rebirth of the Confederate States of America OA strong central government O State s Rights O Promoting the Republican Party

Why was the GI Bill of Rights passed in 1944 O To honor veterans O To allow veterans to study for free and seek higher education degrees To ease the transition from war to civilian life O To find a new job more easily

The government agency that regulates and licenses television and other communication industries is I Federal Communications Commission II Mass Media Commission III National Institute of Standards and Technology OI only O II only O III only O I and II only

Which of the following is not correct concerning President Truman 1 His second term was more successful than his first II Truman failed at creating irrigation projects III Truman was successful in creating over a million low income housing units OI only Oll only O Ill only OI and II only

Who was the Vice President during President Franklin Roosevelt s rule who became the president after his death O Richard Nixon O John F Kennedy O Harry Truman O Woodrow Wilson

What were the two major goals of Truman s presidency rebuild Europe and stop the spread of communism Ostop the spread of communism and restore the American economy restructure the Federal Bank and restore the American economy O rebuild Europe and restructure the Federal Bank

Study the diagram Based on your own knowledge which of these choices would best fit into the center circle Blacklisting HUAC Communist Infiltration O Second Red Scare O McCarthyism O Red Scare O Joseph McCarthy Hollywood Ten R t Q

Look at this diagram During the Yalta Conference it was decided that after the war Germany would be occupied by forces from four different nations Which nation belongs in the empty quadrant United States Soviet Union O Spain O Italy O Russia France Post war Germany Great Britain

General Douglas MacArthur s miracle counterattack in South Korea allowed the Americans to nearly unify the two Koreas before O the Chinese entered the war O the United Nations decided to pull back O Josef Stalin threatened war O President Harry S Truman decided to pull back

President Truman refused to back down from Stalin s closure of western access to this city Warsaw Berlin Moscow in

Which of the following is expressed in the poem Wasteland by poet T S Eliot Negative aspects of postwar life in the world with no moral and spiritual values Positive aspects of postwar life in the world with enhanced moral and spiritual values O Current situation of the world and the plans for improving fast The happenings in the war along with the plans to overcome the bad past

Choose the correct answer The biggest entertainment during the 1920 s and 1930 s was O baseball O motion pictures O live music football

How did the New Deal mark a shift in the U S government s relationship with its citizens and the economy O The New Deal allowed the government to have a more active role in citizen s lives The New Deal made the government become a more active aspect in providing economic stability for the individual O The New Deal gave the government total control of its citizens The New Deal allowed the government to have a more active role in citizen s lives It also made Re Res this Ques 1 5

Choose the correct answer European architects developed a new style of architecture called O Adirondant O Foresquare O International O National style

Who used the concept of Surrealism in his work Franz Kafka a Czech writer O Marcel Proust a French novelist O Sigmund Freud the founder of modern psychology Oswald Spengler a German historian and philosopher

What were the problems faced by France in the years after World War I France s agriculture and industry were destroyed and its economy was weakened Government O expenses rose and the country needed to strengthen its military which had been weakened due to the war The construction of the Maginot Line safeguarded France from German attacks and improved economic conditions The Great Depression led to unemployment in France Additionally the government needed more funds for the army None of the choice 19

How did consumerism affect societies Consumer culture changed people s lifestyles O People began relying on credit to purchase items O People became more focused on the present rather than on planning for the future O All of the choices

The Act of 1935 created a seven member board to regulate the nation s money supply and the interest rates on loans O Social Security O Money O Legal Tender O Bank Res Rese this Ques 1 F 5

850 airports 651 000 miles of road 110 000 libraries schools hospitals Based on your own knowledge the Second New Deal program that built the items on this list was called the Farm Security Administration Agricultural Adjustment Act Works Progress Administration WPA O Public Works Administration PWA t 110 5 9

Spending more money than the government receives in revenue is called O over spending O under spending O shortfall O deficit spending

A former traveling salesman I was a spokesman for the poor and became the Louisiana governor as well as U S senator I proposed a national social program called Every Man a King Who am I O Francis Townsend O Charles Coughlin O Huey Long O Sewell Avery C

Who was the photographer hired by the Farm Security Administration FSA in 1937 to make a pictorial record of the rural areas O Francis Perkins Jackson Pollock O Dorothea Lang O Frances Perkins R Re thi Que 5

The program designed to alleviate the problems of the Great Depression was known as the O Real Deal ONew Deal Fair Deal Square Deal 33 Res Rese this a Quest 1 R 5

A circular molecule has two sites for the restriction enzyme Pst and one site for the enzyme BamH1 How many fragments will result when digested with EcoRI enzyme zero one two three four

Messenger RNA is different from other types of RNA because messenger RNA has a 3 polyA tail introns and exons a 5 methylated cap Both A and C are correct

Which whole blood fraction is the most abundant source of genomic DNA Red blood cells Platelets Serum Plasma

What is the concentration of DNA whereby a 1 100 dilution has an absorbance reading of 0 215 at 260 nm 6 0 ug mL 1075 ug mL 175 ug mL 1750 ug mL

When you look in the mirror you see mostly

What does a spectrophotometer directly measure The weight of a material The amount of light that a substance absorbs What wavelength of light we are seeing The amount of a chemical in a material Question 6 1 point Listen We need to determine the pH of a solution because an incorrect pH can affect the activity and structur biomolecules such as proteins sugars and nucleic acids True

The representation of Beer Lambert s law is given as OD ecd If d represents distance c represents concentration and OD represents absorption what does e represent path length color intensity concentration of the sample extinction coefficient Question 4 1 point Listen The formula for determining DNA concentration is DNA 50 ug mL x OD 260 What is the concentration of a solution with an OD260 of 0 8 45 0 g mL

100 10 1000 0 1 Question 10 1 point 4 Listen A p20 pipette should be used to acquire 21 ul of liquid True False

Beer s law states that as the concentration of a substance in solution becomes greater its absorbance will Decrease Increase Stays the same Cannot be determined Question 2 1 point Listen The OD280 reading of an unknown solution is 1 5 What does this mean The DNA concentration of this solution is 75 g mL This reading is unreliable the solution must be diluted and the OD checked again There is no DNA in this solution