Biotechnology & its Applications Questions and Answers

Griffith s experiment with mice and different Streptococcus pneumoniae species demonstrated this A Conjugation B Transformation Trance

1 pts You cut a plasmid with a restriction enzyme that cuts it into three pieces one fragment is 240 base pairs one fragment is 600 base pairs and one fragment is 1200 base pairs You carry out gel electrophoresis of the digested sample After fifteen minutes which of the fragments will be closest to the top of the gel where the samples were inserted O the 600 base pair fragment O all three fragments will be equally near the top because of their identical sticky ends O the 1200 base pair fragment O the 240 base pair fragment

Bacteria can become resistant to tetracycline in various ways Please match the mechanism of resistance with the most likely genetic variation event 2 4 a tetracycline inactivating enzyme appears that is unrelated to other enzymes in the bacterium tetracycline no longer binds to the ribosome a new tetracycline pump arises that is related to an existing pump in the bacterium extra ribosomal subunits are made to X X horizontal gene transfer gene duplication and divergence a mutation within a gene a mutation within the regulatory DNA of a gene

more difficult to find good antifungal drugs compared with antibacterial drugs why Mode of action many antifungal drugs exploit differences between human and fungal cell membranes Human cell membranes contain cholesterol whereas fungal cell membranes contain ergosterol not cholesterol Examples imidazoles miconazole clotrimazole inhibit ergosterol synthesis Nystatin binds to ergosterol in fungal cell membranes disrupting the cell membrane Antiviral drugs more difficult to find good antiviral drugs compared with antibacterial drug why Mode of action Many antivirals are nucleotide analogs that inhibit viral nucleic acid synthesizing enzymes Examples

29 What has Wade learned at the end of this novel Write at least 100 words in paragraph format

What technique was used to make the grey band become narrower and narrower in the image below HOURS 21 8 5 10 7 12 0 14 9 IZI 19 2 21 3 Chromatography ODNA replication 0000 Density gradient centrifugation O Microscopy

If the intracellular reaction A B C proceeds in the presence of a specific enzyme and no other components you can conclude that A the reaction would not proceed in the absence of the enzyme B the reaction would proceed in the absence of the enzyme but at a slower rate C the reverse reaction A B C would not proceed in the presence of the enzyme D the reaction in the presence of the enzyme will not proceed any faster if the temperature is raised a few degrees

Read the excerpt from a reference article on London then answer the following question The capital of England and the United Kingdom London has thrived for nearly two millennia as a center of commerce and culture Founded by the Romans in about 43 CE the mainstay of the city has always been its proximity to the Thames River allowing it to become a center of trade and maritime power Still more strife arose during Charles I s reign with London at the center of severe political struggles between the king and Parliament London became the refuge of the Parliamentarians and thus the target of royalist forces Londoners helped to rout the royalists at nearby Brentford in 1642 Under Oliver Cromwell Jews began to settle in London as a distinct community they had been banished in 1292 participating in Cromwell s government and attaining English citizenship By the beginning of the 19th century London s population was over one million The hub of the world s economy the city prospered as the British Empire reached its high point of expansion and power under Queen Victoria By the 19th century London could be considered the Hub of the world s economy Defensive center of London World s poorest city

Which two sentences describe what scientists have discovered about red algae and sponges A The earliest fossil evidence of multicellular life is of red algae while the earliest fossils of sponges also multicellular appeared much later B Both red algae and sponges are multicellular but sponges are classified as animals and red algae are not C Sponges evolved before red algae because each cell has a unique job while red algae cells all perform the same job D Biomarker evidence of sponges has been found in rocks that date back to the great oxidation event while evidence of red algae appeared long after the event

regulation OA Both control whether RNA polymerase can bind to and transcribe genes B Both use enhancers far from the gene to regulate the rate of transcription C Both have proteins that can bind to mRNA and prevent translation OD Both regulate protein production at all stages of transcription and translation

Amos works for a medical device company and was tasked with creating new educational material to market the device He is deciding between two statements to 5 points describe the device risks including 20 out of 100 people will die from the device or 80 out of 100 people will be saved by the device If getting the device is considered the risky treatment option which is the best message Amos could choose a Amos should select the statement describing how many people will die because negative framing often leads people to choose more secure options b Amos should select the statement describing how many people will die because negative framing often leads people to choose riskier options c Amos should select either option that he likes because the wording is unlikely to affect how many people decide to get the device d Amos should select the statement describing how many people will be saved because positive framing often leads people to choose riskier options Save Answe

A negative option plan is a marketing device through which the consumer responds to the seller only if she wishes not to receive the product True False

The Federal Trade Commission can order a company to remove or modify a deceptive trade name True False

2 In the clinical trials for a new diagnostic antibody test the experimental test had 32 of the undiseased test group test positive for the disease Based on this what can you infer about the test s specificity Anguer

First step Place a microscope slide on the benc Submit Place the prepared slide on the microscope stage Previous Answers Request Answer Put one or two drops of the sample Take a coverslip and place it on top of from the beaker onto the slide the sample Last step Locate the organisms X Incorrect Try Again One attempt remaining What constitutes a prepared slide that is ready to be placed on the microscope stage Make sure you have placed the steps preparing the slide before the step placing it on the stage

questions Place the labels on the image of a compound light microscope Group 1 labels identify the parts group 2 labels identify the functions Stage clip Coaxial stage controls Move the slide right left and backward forward Secures the slide on the stage Group 1 Group 2 w Group 1 Group 2 Reset Help

Question 8 Cartoon and movie characters such as Wolverine Mystique and Storm lead to the notion that genetic mutations always cause profound changes in the phenotype of an organism However the lesson over DNA and Genetics clearly shows that mutations may or may not have an effect Which of the following mutations would most likely NOT cause an effect in an organism A Codon chart has been provided below to assist you O TACCTCATC TACCTCATC U L C A Mars TACCTCATC TACCTGATC G TAGCTCATC TACATCATC Code UUU UUC UUA UUG CUU CUC CUA CUO AUU AUC AUJA AUG CUU CUC GUA GUG U G Anso ACI CODE ARVO ACRS COD2 ATOS ACE COON ART ARD phe UCU UCC UCA UNU tyr UAC UAA STOP JAD STOP UCO CCU CAU CAC his CCC CCA CCO ACU CAA CAG AAU AAC ACC leu leu de met val ACA ACG GCU occ Second Posion GCA GCG ser pro the da AAA AAG GAU GAC GAA GAG gin asn lys asp glu UGU UGC UGA UGO CGU COC COA COO AGU AGC AGA AGO GOU GGO GGA GGG cys STOP trp ard ser arg dy U CANDUAGOA Third Position G

every day had a 61 higher risk avoided diet drinks For this study Hannah Gardner about their eating behaviors exercise habits as well as cigarette and alco also given physical check ups including blood pressure measurements and blood tests for cho other factors that might affect the risk for heart attack and stroke The increased likelihood of vascular events remained even after Gardener and her colleagues accounted for risk factors such as smoking high blood pressure and high cholesterol levels The researchers found no increased risk among people who drank Question The findings of this study suggest that consuming diet soda might lead to increased risk for heart attacks and strokes From the statements below identify additional evidence that supports this claim Findings from an epidemiological study suggest that NYC residents are 6 8 times more likely to die of vascular related diseases compared to people living in other U S cities Results from an experimental study demonstrated that individuals randomly assigned to consume one diet soda each day were twice as likely to have a stroke compared to those assigned to drink one regular soda each day Animal studies suggest a link between vascular disease and consumption of caramel containing products ingredient that gives sodas their dark color

X Biology Area D Asses Time Limit 0 35 00 8 12 6 ww 3 Time Left 0 30 09 10 Sara Jah Lymphoma Carcinoma Adenoma Luna CSarcoma No tumor 73 18 Tumors found in type A and type B mice Pie chart depicts relative incidence of tumors Numbers outside each slice denote the percentage of specific tumor type 1 Modified from Wang Y S Klumpp H M Amin H Liang J Li Z Estrov P Zweidler McKay S J Brandt A Agulnick L Nagarajan 2010 SSBP2 is an in vivo tumor suppressor and regulator of LDB1 stability Oncogene 29 3044 305 Type A mice with Lymphoma were more common than type A mice with no tumors Type B mice were more likely to have tumors than type A mice Lymphoma was equally common among type A and type B mice Carcinoma was less common than Lymphoma only in type B mica

QUESTION When beetles were introduced as predators to the Leopard frog tadpoles and the pest Malathion was added the results were unusual Which of the following is a plausible hypothesis to explain these results The Malathion killed the tadpoles causing the beetles to be hungrier and eat more tadpoles The Malathion killed the tadpoles so the beetles had more food and their population increased The Malathion killed the beetles causing fewer tadpoles to be eaten The Malathion killed the beetles causing the tadpole population to prey on each other

Litera Microscope lens should be cleaned with soap and paper towels True False Question 6 1 point 1 Listen Where are the objectives located

Listen What is the total magnification for an image that magnified by a 10x ocular lens and 4x objective Ax Question 3 1 point Listen tmont knob is used to focus on objects at high power

9 What does the field of biology study O A Living things OB Insects and arachnids OC The mind and how animals learn O D Elements and compounds

Which one of the following statements is true DDT does not help prevent disease from passing from agricultural animals to humans Cost was a major factor in the United States government s decision to ban DDT Many African governments concluded that the potential long term health effects of DDT were not as serious as the immediate problem of insect control DDT cannot accumulate in the fat of animals The DDT ban in the United States has made it very difficult to control agricultural insect pests

A complex tone composed of a 440 Hz tone an 880 Hz tone and a 1320 HZ tone is presented What is the fundamental frequency 440 Hz 880 Hz Othere is no fundamental frequency 220 Hz

Where are MHC Il proteins found and what cells recognize them all body cells Thelper cells antigen presenting cells APC Thelper cells antigen presenting cells APC T cytotoxic cellis all body orille Toittedrar aille brads

2 The table below shows partial diploid E coli with lac operon mutations 15 pts For each mutant indicate whether expression of beta galactosidase is None absent Basal on but low expression High on and activated expression when grown in either lactose only or glucose only media Also indicate the cell s overall growth phenotype lac or lac when grown in lactose only and why you expect that phenotype provide why for ONLY THREE of the mutants Recall that a bacterial cell needs at least one functional copy of lacZ AND lacY expressed at high levels to produce an overall growth phenotype of lac when grown in lactose only media Lac Mutant I P O ZV Why I P O Z Y I P O Z Y CAP binding Expression in lactose only site T WT Expression in glucose only Phenotype in lactose only

Match each type of water pollution with its description Nonpoint Source

Why does Jason Stanley feel optimistic about this snail invasion They are only in one area that is already being treated Dogs like Mellon have tracked down most of the snails Doctors can easily treat patients with meningitis O Florida is spending 23 million to get rid of the snails

Genetically modified organisms GMOs have included corn crops engineered to produce a natural pesticide to kill a certain insect which would normally destroy the corn The benefits include a larger corn crop because the destructive influence of the insects is owered What might be one negative result of growing this specific GMO OA The natural pesticide could get into the soil and kill the next corn crop OB All of the insects could disappear allowing the corn crop to grow too large OC The genetically modified corn would require more water to produce enough pesticide to fend off the insects OD The insects could grow resistant to the pesticide in the genetically modified corn and become immune to regular pesticides

Problem Our current energy sources are not sustainable and are causing increases in carbon emissions which contribute to global climate change We need to find alternative energy sources that are more sustainable for the environment but are also economically viable Directions In order to pick the best solutions for alternative energy sources you will need to use a cost benefit analysis There are advantages and disadvantages to each solution and different solutions can address different parts of the problem Use this assignment to demonstrate your ability to evaluate competing design solutions Here is a link to the Crops Overview Slideshow Use the slideshow to describe the nature of the problem each solution addresses Corn READ THE SLIDES AND GET THE INFORMATION FROM THERE Switchgrass Miscanthus Native Grasses Use the slideshow and critical thinking skills to identify the evidence Why does society need the BEST solution

Describe the strengths of each solution Corn Switchgrass Miscanthus Native Grasse

uppose that 45 mL of water was added to the acids in Experiments 1 and 2 instead of 85 mL How would this affect the results of the experiments The results would be falsely high O It would have no effect on the results The results would be falsely low The end point could not be determined

Controlled burning is the intentional introduction of light fires into forest regions What is the main benefit of controlled burning for human populations O A It stops the cycling of nutrients OB O C OD It decreases the rate of deforestation It reduces the likelihood of major forest fires It destroys weeds and other introduced species

A fish species with little diversity exists in a single pond A long term drought causes the pond to dry out What is the likely result of natural selection in response to this environmental change A The fish species will evolve into a species that can live on land B The diversity of the fish species will increase C There will be an increase in the fish population D The fish species will go extinct

How can the bacteria growing on this Sheep s Blood Agar plate be classified Gamma hemolysis Alpha hemolysis

The first commercial application of genetic engineering was the use of bacteria to make insulin a medicine needed by many people with diabetes Before the use of genetically engineered bacteria insulin had to be harvested from the pancreases of slaughtered animals How has genetic engineering most likely improved the lives of diabetics OA It has made insulin cheaper and more readily available OB It has made the incidence of diabetes decrease It has made the demand for insulin decrease OD It has made it easier for diabetics to inject themselves with insulin O C

The legally recognized status of someone within a nation is known as O political party affiliation O citizenship O diplomacy

70 Nearly all peptide bonds are in the trans configuration because A trans peptide bonds are stronger B cis peptide bonds prevent R groups from interacting C cis peptide bonds are weaker D trans peptide bonds minimize steric hindrance of R groups 70

Nitrogen atoms are part of the structure of some organic molecules such as all amino acids and some modified carbohydrates What other organic molecules contain nitrogen O A fatty acids OB O C starch nucleotides OD cellulose

the correct answers Which of the following is true about nucleic acids Glucose cellulose and starch are examples of nucleic acids found in most cells Both DNA which stores genetic information and encodes protein sequences and RNA whic is involved in the direct production of proteins are nucleic acids Nucleic acids are usually insoluble in water and are used for long term energy storage Nucleic acids always consist of a five carbon sugar a nitrogenous base and one or more phosphate groups

Drag and drop the terms to complete the history of the study of DNA 1 ONA was first isolated from white blood cells by 2 3 Avey MacLeod and Experiments by 4 who called it nuclein because it was isolated from nucle experimented with strains of Streptococcus pneumoniae providing the first evidence that DNA may be the transforming proved that DNA is required for the transformation of bacteria and Chase using bacteriophage 12 proved that DNA is the genetic material found that the ratio of A Tand C G and that the percentage content of A TG and Cis different for different species

Which of the following is NOT a step in enzymatic catalysis A Substrates enter the active site B Products are released Substrates are held C at the active site D The active site breaks down

What can biodiversity influence within an ecosystem well the ecosystem grows after rain how poorly the ecosystem responds to a drought how well the ecosystem recovers from a disturbanc

17 Record your results in the following Table 2 Table 2 Band 1 2 3 4 Distance traveled mm 2 3 Table 3 Band color Distance solvent front moved 18 Calculate the Ry values and record in Table 3 Molecule beta carotene yellow xantho phun bright gran chorphyll a yellow grown b youllow orange beta carown mm Identity R xanthophyll chlorophyll a chlorophyll b 19 What factors affect how pigments get separated during chromatography 20 If you used a different solvent for this experiment would you expect the Rf value to be similar or different Explain 21 What caused the pigments to separate during the chromatography procedure

top predator carnivores herbivores producers Energy Herbivores In the energy pyramid here which organisms have the most energy available Carnivores Producers Top Predator

The pH of skin that is believed to discourage the growth of many microbes is 6 to 7 4 to 7 3 to 5 6 to 8 3 to 8

Phenol red V 9 Half fill a 1 Liter beaker with ice 10 Place beaker 3 in the 1L beaker filled with ice 11 Record the initial color of the solution in Table 2 then check and record any observations every 30 minutes for 2 hours Record the initial color and your color changes in the result table below 12 When you have completed this experiment please return the beans to their respective containers MAKE SURE THAT THE NON GERMINATING BEANS STAY DRY During the incubation period answer the following questions 1 Write a hypothesis for this experiment I belive that ob react more Phende color will with the bume z 2 What is your independent variable This will be based on your hypothesis 3 What is your dependent variable This will be based on your hypothesis 4 Name one standardized variable in this experiment tan yellow

If you are trying to achieve cardiorespiratory fitness what intensity are you working at O Enough to increase muscle strength O at 55 90 maximum heart rate O at 25 50 of maximum heart rate enough to develop range of motion

Match the testing method to the purpose Agarose gel electrophoresis RFLP Microarray Technology Cho Cho Analy Dete Ident Dete It is u Math