Biotechnology & its Applications Questions and Answers

Use the following information to answer the question An mRNA molecule with the sequence 5 CCG ACG 3 is being translated at a ribosome The following charged transfer RNA molecules with their anticodons shown in the 3 to 5 direction are available Two of them can correctly match the mRNA so that a dipeptide can form tRNA Anticodon GGC CGU UGC CCG ACG CGG Amino Acid Proline Alanine Threonine Glycine Cysteine Alanine Which of the following dipeptides will form from this mRNA O glycine cysteine O alanine alanine O proline threonine O cysteine alanine

Use the following information to answer the question In an effort to produce large amounts of a eukaryotic gene product a plasmid that contains both a eukaryotic gene of interest and an antibiotic resistant gene was constructed A transformation was performed to attempt to insert the plasmid into the bacterium E coli Which of the following describes a growth condition and culture analysis that supports the claim that plasmid has entered the E coli bacterial cell A B C D OA C OD Growth condition Water plus antibiotic Nutrient broth Nutrient broth plus antibiotic Nutrient broth plus untreated bacteria Culture analysis Protein of interest is detected Plasmid is detected in media Bacterial cells multiply Lysogeny is induced

Which of the following correctly lists the processes in order for one cycle of polymerase chain reaction PCR denature DNA add fresh enzyme anneal primers add dNTPs extend primers O denature DNA anneal primers extend primers O anneal primers denature DNA extend primers O extend primers anneal primers denature DNA

Which of the following sequences is most likely to be cut by a restriction endonuclease O 5 AATATT 3 3 TTATAA 5 O 5 AATTCT 3 3 TTAAGA 5 O 5 ACTACT 3 3 TGATGA 5 O 5 AAAATT 3 ini TITTA A F

ert the following A 98 6 F to Celsius B 35 C to Fahrenheit

sequences Reverse Repetitive Restriction Helicase O Recombinant enzymes are proteins that cut DNA at highly specific bas

number the following steps for soft lithographic stamp generation blanks are and NOT letters one stamp micro patterns for controlling cells spin coat a polymer photoresist set a photo mask develop the substrate expose the substrate take a Si substrate

What do you think would happen if plants and animals were placed in the same test tube 1 mark Answer

with that of suspects is clone A genetically modlifd organisms B Genetic engineering C ONA extraction D Gel Electrophoresis using

1 Typical catalytic rate of enzyme carbonic anhydrase is 10 3 per millisecond How many molecules can carbonic anhydrase hydrate per second and what are these molecules A 10 3 molecules of water B 10 6 molecules of carbon dioxide C 10 3 molecules of carbon dioxide D 10 6 molecules of carbon oxide E 10 5 molecules of oxygen

This fingerprint was developed using which latent print developing technique

Sample 1 Sample 3 2 Disk Sample 1 Sample 2 PE ME CE VA PE ME CE VA Sample 3 Sample 4 PE ME PE Part 4 Conclusions ME CE Zone Size Madeline s Mother Madeline s Sister Madeline s Father Delivery Room Surface Sample 3 4 PE ME Sample 4 PE ME Disk PE ME CE VA PE ME CE VA 3 6 The following table identifies the sample sources Which sample contains MRSA How do you know VA CE Zone Size I did question 6 plz do 7 and 8 7 Sample 2 was taken from a nasal swab of a family member who has been having sinus infections What course of antibiotics would you recommend 8 What recommendations would you make to Madeline s family and the hospital where Madeline was delivered Your recommendations should include evidence based reasoning and details from the case to

What can you do to get those bonding arms to match up with each other

What are some of the strengths of BLASTp searching and Conserved Domain searching for protein annotation O Conserved Domain gives back whole protein sequence OBLASTp can assign potential function to a protein Conserved Domain can assign potential function to a protein OBLASTp gives you all of the potential domians of a protein OBLASTp gives back whole protein sequence

What would happen to a protein like signal peptidase if you removed its signal peptide but added a nuclear localization signal NLS It would end up in the nucleus It would end up in the cytoplasm It would be secreted None of the above

Which of the following best describes lactose presence or absence in the bacterial growth medium OA If the bacterial medium contains glucose and lactose the bacterial cells will utilize glucose instead of lactose to generate energy CB If the bacterial medium lacks glucose and lactose is present the bacterial cells will break down lactose into glucose and galactose including alolactose and the bacterial cells will utilize the glucose to generate energy OcIf the glucose in the bacterial medium is exhausted and lactose diminished the lac repressor protein will bind the operator to prevent transcription in bacterial cells D Answers A B and C are the correct answer choices for this question E None of the answers is the correct answer choice for this question

What can we assume about this society based on this scene SELECT AN ANSWER O Husbands frequently try to poison their wives O Many people are dissatisfied with their lives O Most people are satisfied with their lives O Medical doctors are revered like gods SUBN

extra credit bacteria that are positive for the oxidase test are most likely to have which type of oxygen requirement aerotolerant anaerobe obligate anaerobes bacteria that are harmed by oxygen facultative anaerobes or obligate aerobes bacteria that cannot do aerobic respiration

You are looking at an MSA mannitol salt agar plate The area of the plate media around the bacterial streak is yellow This tells you O bacteria ferments mannitol bacteria cannot ferment mannitol bacteria cannot ferment glucose bacteria cannot ferment lactose bacteria ferments lactose bacteria ferments glucose

You inoculated some bacteria into a tube of fermentation phenol red broth the broth turns yellow and you see a large bubble that fills the little tube inside ie the bubble empty space extends beyond the tip of the little tube This tells you sugar in the tube was fermented into acid and gas NO fermentation took place the sugar in the tube was fermented into alcohol only sugar in the tube was fermented into acid ONLY O sugar in the tube was fermented into gas ONLY

used in laser scanning confocal microscopy improves the quality of the image by O Lowering the limit of resolution O Decreasing the size of the field of view O Eliminating out of focus fluorescence O Decreasing the working distance QUESTION 23 Imagine you need to pipet 0 015 mL of enzyme stock solution into an enzyme assay What volume is this in L and which micropipettor must you use 150 L p200 1 5 L p20 15 L p200

Question 10 Points 2 The direction of current in the Fleming s left hand rule is given by O thumb O middle finger O index finger O little finger

Question 1 The flow of charge through a wire is called Points 1 electricity magnetism O heat light

needs to be done in an aqueous solvent 25 26 T F A Sepharose gel filtration matrix is made up of agarose beads IT E The number 150 in Sonhad 50 m so that it has and evolucion

A ball with a mass m strikes a wall horizontally with the velocity v and rebounds back with the same velocity What would be the impulse imparted by the wall on the ball O mv O 2mv O 3mv O mv 2

Question 7 Points 1 Which of the following is an example of inertia A football lying still on the ground until it is kicked Falling forward when the bus in which you are traveling comes to a sudden halt Falling backward when your car starts to move from rest O All of the choices

T F Sephadex G gels are types of ion exchange resins 11 After a TLC run an experimenter determines that two spots that migrated the same distance are not the same compound The discrepancy could be due to 10

19 In gel filtration chromatography the molecular weight limit for entrance into the gel matrix is known as the limit 20 T F If you need to store a gel filtration column for one or two weeks in between runs it is best to remove all buffer from the column and store the gel dry

13 T F A Sephadex G 75 column will not be able to separate proteins that have a molecular weight higher than 300 000 daltons 14 T F An important consideration in getting a good separation of components of a mixture by TLC is spotting the TLC plate with an appropriate concentration of the mixture

9 T F The best resolution on a TLC chromatogram depends upon how well you apply the sample 10 T F Sephadex G gels are types of ion exchange resins

Which of the CRISPR Cas9 mediated edits might do an even better job of restoring function O An insertion of 3 bases within exon 23 A deletion of 9 bases within exon 23 O A deletion of 10 bases within exon 23 O Use of homology directed repair to replace the mutant codon with the wildtype codon Either B or D

Question 6 Points 2 Identify the kinematic equation which relates the displacement and time O v 2 2a x xo O x xo vot 2at Ov vo at x xo vot 1 at

Question 5 Points 1 The slope of the velocity time graph of a car represents the acceleration of average velocity of distance covered by time spent by Complete Later Complete the car

Velocity v m s 80 60 40 20 0 A 0 10 30 Time t s Which of the following best describes the motion of the cyclist B 20 40 C 50 60 The cyclist steadily increases his speed to 40 m s in 10 seconds and he retains the speed the 48th second and then increases to 80 m s The cyclist steadily increases his speed to 20 m s in 40 seconds and he retains the speed the 50th second and then increases to 60 m s O The cyclist steadily decreases his speed to 20 m s in 40 seconds and becomes zero

One of the main problems in the early growth of cities was O a lack of planned development O a shortage of workers O child labor O destruction of forests

6 Type the correct answer in the box Spell all words correctly Which book asserted that due to the expanding human population the ecosystem will collapse by the middle of the twenty first century Dennis Meadow s of the twenty first century asserted that due to the expanding human population the ecosystem will collapse by the mid Reset Next

Select the correct answer from each drop down menu Which is a step of the scientific method in environmental science is a step in the scientific method The step that follows this step involves forming

20 pts In chapters 16 and 17 we learn more about function of DNA as well as what happens when things go wrong such as mutations or telomere loss We learn about a few scientists in these chapters and the incredible experiments they conducted examples such as the Hershey and Chase experiment Rosalind Flanklin s X ray crystalography and Beadle and Tatum s amino acid synthesis mutation experiments But there are so many more incredible scientists who ve contributed to our knowledge and we simply can t fit them all into thte textbook Please look up one of the people below or find your own and share what contributions they ve made and something you found interesting about them Marie Maynard Daly Barbara McClintock Henrietta Lacks Alec Jeffreys Robert Waterston Oswald T Avery Phoebus Levene Thomas D Brock Nettie Stevens Elizabeth Blackburn

An autotrophic microbe with a nucleoid is Oan algae O a fungi a phototrophic bacteria a slime mold O a plant

2 What is the volume of a 2 5kg block of wood if wood has the density of 0 8 g cm

Question Evaluate how well the refinement you suggested will affect the stability of the natural system being impacted and the feasibility of the refinement cost reliability available technology research

Question Identify the scientific knowledge and reasoning on which using biofuels instead of fossil fuels is based Provide both the quoted evidence and the source reference s USE COURSEARC OR OTHER SOURCES TO FIND EVIDENCE REASONING TO SUPPORT THE USE OF BIOFUELS

Question Describe how using biofuels may help in stabilizing the natural system you identified as being impacted

Which statement best describes how the research of Hutton and Lyell relates to the theory of evolution OA Geological forces that cause biological changes must be very powerful to have an effect B Changes to landforms and living things are unobservable showing that Earth has been mostly unchanged OC Biological changes occur much more rapidly than geological changes OD Changes in landforms and living things both occur gradually over long periods of time

A A null hypothesis of There is no statistical difference between two sets of data must be to show that there is a statistical difference correct B close C void

Choose the correct answer Countries which provide extensive social services at little or no cost to the users are welfare states Ofree states O social states O highly taxed states Complete Later Complete

Points 1 Question 4 What type of government operates in Great Britain O Monarchy Constitutional O Constitutional monarchy O Republic

What is the name of theory that describes sap transport through xylem Describe this theory including the relevant cell types that are involved in sap water transport Base your answer on the cell types and processes as occur within most angiosperms Chapter 30

A simple microscope consists of a single Odouble three lens es

DNA fingerprinting can be used to A resolve paternity disputes Mark all that are correct B identify body parts in mass disasters C determine the source of contaminated food like romaine lettuce D solve crimes that have blood or other body fluids left behind at the crime scene or on the victim