Biotechnology: Principles and Processes Questions and Answers

of the following that are true of outer hair cells in the cochlea Impulses from outer hair cells in different positions along the basilar membrane are interpreted as specific pitches They can decrease the motion of the basilar membrane to protect inner hair cells from loud noises They can contract and stretch which affects the stiffness of the basilar membrane They can increase the ability to distinguish small differences in pitch by amplifying the motion of the basilar membrane Question 11 2 points 4 Listen Match the following sound wave properties to their descriptions DE The height of the crests The distance between two consecutive crests How we perceive different frequencies of sound waves The number of waves that pass a given point within a 1 Amplitude 2 Frequency 3 Pitch 4 Wavelength

Make a two column folded chart Label the front Types of Microscopes and lab the inside as shown Use it to organize your notes about microscopes

Fermentation Worksheet Type in the blanks and submit this worksheet through the assignment tab in iCollege Delete the words Insert Picture Here before inserting each picture Sugar Source I Chose Was Insert a picture of your warm water fermentation vials below Remember to include a name card

Which human activity is likely to cause damage to an environment OA Using native species for ground cover B Using water efficient irrigation for agriculture OC Preserving regions in a rain forest OD Clear cutting a forest for development

If a neurotransmitter binding to its receptor opens Cl channels which of the following would occur an EPSP an action potential presynaptic inhibition an IPSD

Within a double stranded DNA molecule adenine forms hydrogen bonds with thymine and cytosine forms hydrogen bonds with guanine What is the significance of the structural arrangement It determines the tertiary structure of a DNA molecule It allows variable width of the double helix It permits complementary base pairing It determines the type of protein produced

How does extracellular glucose inhibit transcription of the lac operon by strengthening the binding of the repressor to the operator O by inhibiting RNA polymerase from opening the strands of DNA to initiate transcription by weakening the binding of the repressor to the operator by reducing the levels of intracellular CAMP

What does it mean to say that a signal is transduced O The signal is amplified such that even one signal mol cule evokes a large response O The physical form of the signal changes as it passes from the cell membrane to the ultimate intracellular target The signal enters the cell directly and binds to a receptor inside The signal triggers a sequence of phosphorylation events inside the cell

Under what conditions does the trp repressor block transcription of the trp operon when the repressor is not bound to tryptophan when the repressor is not bound to the operator Owhen the repressor binds to tryptophan when the repressor binds to the inducer

In order to improve the memory of a healthy or mutated mouse design an experiment where you will be using optogenetics to induce an LTP that results in memory consolidation The experiment should include which opsin will be expressed and at which region of the brain Also explain the behavioral test that will be done to test if the experiment really induced the LTP and improved the memory of the mouse Explain how LTP will be generated by the technique and what are the expected roles of calcium and CREB the induction of the early and late LTP

A difference between amphibians and reptiles is that Oamphibians are invertebrates but reptiles are vertebrates Oamphibian eggs can survive out of water and reptile eggs cannot amphibians have thin skin whereas reptiles have thick skin covered with sca Oreptiles are considered tetrapods whereas m

Invertebrates are defined as animals that lack O O an exoskeleton an endoskeleton O a backbone a head

Select all that correctly apply to the human pedigree shown below Individual 2 is heterozygous The pedigree represents dominant inheritance Individual 3 is heterozygous The pedigree represents recessive inheritance The pedigree represents x linked recessive inheritance

The diagram below shows a bacterial replication fork and its principal proteins Drag the labels to their appropriate locations in the diagram to describe the name or function of each structure View Available Hint Coats single stranded DNA Breaks hydrogen bonds unwinding DNA double helix Synthesizes RNA primers on leading and lagging strands Synthesizes DNA 5 to 3 on leading and lagging strands Replaces RNA primers with DNA nucleotides Catalyzes phosphodiester bond formation joining DNA fragments Relaxes supercolled DNA Leading strand Lagging strand Group 1 Group 1 Group 1 Group 1 Group 2 Group 1 Group 1 Group 1 Overall direction of synthesis Group 2 Reset Help

Liverworts hornworts and mosses are grouped together as bryophytes Besides not having vascular tissue what do they all have in common A They are all wind pollinated OB They are heterosporous O C They require water for reproduction O D They can reproduce asexually by producing gemma

The greater color change in the bromothymol blue experiment when running showed an increased rate of production A glucose C oxygen B carbon dioxide D water

A The electron transport chain is a series of diffusion facilitating membrane proteins found in the membrane of the mitochondria outer surface of the finer

Glycolysis involves two phases investment and energy energy B first C

A Step 1 Step 2 C C C C C C After step 2 the next aerobic step is the citric acid cycle which will lead to the release of as waste C C C C C C 0 B H O C CO

Reactions that require energy input are called endergonic and are A anabolic B catabolic C easily activated D hard to activate

1 1kbp Hindill 0 15 kbp Oc3 O d 4 O e 5 Hindill amp 0 25 kbp 1 6 kbp EcoRI How many DNA fragments will EcoRI produce from the plasmid shown in Figure 1 Oa 1 Ob 2

The sticky ends of the molecule below suggest that this molecule was cut by C O a reverse transcriptase O a DNA polymerase O a restriction enzyme a ligase LACE

The unusual formations in this segment of DNA are called dimers Ultraviolet UV radiation from sunlight among other things can damage DNA by causing the formation of thymine dimers Thymine dimer G T T GCT CGA CAA G Ultraviolet light GTA CCAT In this image the presence of ultraviolet light has caused damage to one strand in a molecule of DNA Which statement best reflects the result of this damage 3 of 6 QUESTIONS Replication of the DNA shown will transmit damaged DNA to random

Figure 1 1 1 kbp Hindill 0 15 kbp Hindi amp 0 25 kbp EcoRI EcoRI 1 5 kbp In Figure 1 after digestion with the appropriate restriction enzyme what is the smallest piece containing the entire ampicillin resistance amp g O a 0 17 kbp O b 0 25 kbp O c 1 08 kbp O d 1 50 kbp O e 3 00 kbp

Question 20 You add DNA for making the color pink to a culture of bacteria pink They must have taken up the DNA via O Conjugation O Transduction O Transposon Transformation

tion 36 ion 35 lls You modify a deadly pathogen to have no antigens on the cell surface What is the predicted host immune response O The response becomes much weaker as the pathogen is no longer easily recognize and destroyed O The response becomes much stronger as the pathogen is now easily recognized and destroyed O The response becomes much stronger as the pathogen has had all pathogenicity factors removed O The response becomes much weaker as the pathogen has had all pathogenicity factors increased ed cells are ordered to kill themselves by

sample double stranded DNA and transcribe mRNA from this DNA Then you analyze the base composition percentage of each base found in each strand for each the two DNA strands and the one mRNA strand Numbers in the table below indicate the percentage of each base in the strand Note results may differ from the true value by about 1 that is the best estimate that the machine you used can make A STRAND 1 40 1 STRAND 2 21 5 9 5 STRAND 3 40 0 29 0 Questions G 28 9 b Which strand would have the C 9 9 29 9 9 7 a What nucleic acids make up strand 1 Select T 0 0 39 1 21 3 U 21 1 0 0 0 0

Choose the list that contains only molecules that are involved in DNA replication in a cell Note that this cell has not been infected with any viruses O Helicase DNA gyrase sigma factors transcriptase DNA polymerase helicase RNA primase and ligase O RNA polymerase tRNA ribosomes and mRNA Reverse transcriptase RNA primase ligase and Okazaki fragments

EXPECTED RESULTS 1 In the environments you sampled which will have the greatest amount of growth on the nutrient agar plates H has the greates amount of growth Do you expect turbidity in the unsterilized nutrient broth that was incubated 3 Will nutrient broth or nutrient agar provide more information on the variety of bacteria in an environment 2 RESULTS Fill in the following table with descriptions of the bacterial colonies on your plates and your classmates plates Use a separate line for each different appearing colony Area sampled Incubated at 32 C for days Estimated Diameter 2cm Whole Colony Appearance I colony Colony Description Margin Undulate Elevation Pigment Convex y ellow Number of This Type

The promoter region upstream of a gene provides a binding site for DNA polymerase RNA polymerase telomerase methyl groups a repressor protein

1 What is the advantage of using Petri plates rather than test tubes in 2 What are bacteria using for nutrients in nutrient agar What is the purpose of the agar CRITICAL THINKING Did all the organisms living in or on the environments sampled grow on your nutrient agar Briefly explain atio of different

tion 5 Certain glass objects are not meant to be heated and could shatter if exposed to a heat source What tun

What does a transgenic organism have that a non GMO clone does not 4 of 6 QUESTIONS The ability to acquire new genetic traits over its lifetime The ability to pass on traits acquired over its lifetime to its offspring ODNA that is identical to that of another organism ODNA from two different species

Question 3 1 point Listen The x helix and B pleated sheet are examples of which level of protein structure A primary OB B secondary C quaternary D primary and tertiary n Browser E tertiary

What are the three main approaches to the identification of unknown bacteria Check All That Apply genetic radiologic immunologic phenotypic

What plane is labeled as 3 4 5 N 2 3

Calcium s atomic number is 20 It forms ions with 18 electrons What is the electrical charge of a calcium ion View Available Hint s 0 2 O 2 O 1 0 1 Submit 13

A scientist wants to modify the DNA of a plant so that the plant expresses a gene that will allow the plant to become resistant to certain plant viruses Which of the following tech niques could the scientist use to modify the DNA of a plant A bacterial transformation B C reproductive cloning gel electrophoresis

A police officer is Investigating a crime scene when she discovers a drop of blood on the window where the criminal broke the window of a house open The officer has five differ ent suspects Which of the following techniques will visualize if the DNA in the blood sample from the crime scene matches the DNA found in the suspects A B C PCR reproductive cloning gene cloning

An alternative to antibiotic resistance is the gene which confers bioluminescence A PGLO B R C ampR DS

Modeling Population Size You have learned about how to sample a population and estimate size from density In this activity you will construct a model of a population sample the population to determine its density then use its density to determine its size Part One Construct the Model Materials 1 piece of 8 5 by 11 in letter paper 1 pen or pencil 1 ruler 50 small dry beans or other small objects such as buttons 1 small cup Directions 1 Use your pen or pencil and ruler to divide the paper into four equal sections 2 Place the 50 beans into a small cup 3 Scatter the beans as evenly as possible over the entire paper making sure to get some of the beans into each section

A transformed bacterium with the ampR gene can easily be tested with an confirming transformation success A antibiotic B a radioisotope C ampR plasmid D tagged phago

Conjugation is a method of bacterial A mitosis B transduction C asexual reproduction D genetic recombination

A Bacteria reproduce which differs from viruses that have a B C Jog

The promoter will be located the DNA that will be read A downstream from B upstream from C 2 base pairs away from D in an organelle away from

What is the purpose of a bacterial promoter in a plasmid O This allows the plasmid to replicate when the bacteria cell divides O This is used to make a transgene This is the sugar found in LB agar plates that allows for expression of plasmid genes This is used to express the gene that you are interested in studying

Which of the following is FALSE regarding generics O O Generics have the same active ingredients as the brand name Generic names may be the chemical name or a term that refers to the chemical makeup of the drug Generics are the trade names of each drug Generics offer savings to patients over the brand name product

What is the function purpose of the guide RNA in the CRISPR Cas9 system

When the Lords of Trade established the Dominion of New England their purpose was to O grant greater local autonomy to the colonists centralize the authority of the Crown O rid New England of witches abolish slavery in New England

Changes brought about by the agricultural revolution included O the possibility of a food surplus the possibility of settlement in one place O technological development O all of the above O only a and b