Biotechnology & its Applications Questions and Answers

13 Complete the tables below to show how the quantity produced and the quantity purchased change in a typical market On the lines below the tables describe what might happen if consumers were willing to pay a high price for a new sustainable product such as a very energy and water efficient yet expensive washing machine Price Low Low Supply Quantity Produced Price Low High Demand Quantity Purchased

Which experiment would most likely contain experimental bias OA A lab performs a test by giving bacterial colonies the same amount of different antibiotics and growing them under the same conditions OB A chemistry class performs an experiment in which each reaction has the same amount of starting material and begins at the same temperature OC A student tests the attraction of bees to flowers by placing four different flowers in the same location and counting how many bees visit each OD A company that makes pain relief medication tests the effectiveness of their own medicine compared to that of three other brands

Part II Techniques for studying gene expression and DNA protein interactions Total 14 marks 1 Complete the table 6 marks 0 5 marks for each box Method ChIP Western Blot EMSA Northern Blot Microarray What is it used for Identifying and separating protein of interest from a mixture What is the target of visualization DNA or RNA What is the readout Activity of gene active or

Which statement describes a disadvantage of gene therapy It can stop cancer cells from growing O It uses viruses that are deadly It can block a mutated gene from working 6 of 6 QUESTIONS

A cell can develop into any type of cell in an organism whereas a cell can become any type of germ layer

The institution of slavery both denied specific gender roles for black men and women and utilized the True False

By the middle of the nineteenth century serialized fiction women men immigrants children comprised the majority of authors and readers of

During the 1990s which politician sponsored the High Performance Computing Act that enabled the Internet to be created A Al Gore B Bill Clinton C George H W Bush D George W Bush

Pure Culture A plate of Staphylococcus aureus with a fungal colony your standing of pure mixed and contaminated cultures Contaminated Culture Mixed Culture

Part A What is an ion View Available Hint s an atom that is sharing electrons with another atom Oan atom that has either gained or lost electron s Oan atom that loses all of its protons Oan atom that has lost one or more neutrons

Part A How many valence shell electrons does the element carbon have H H H two four Six Oeight H D

A researcher is trying to determine how to stop the transcription of a gene found in hu mans involved in cell growth The researcher wants to stop the binding of RNA polymerase to the DNA sequence to stop transcription of the gene from occurring If the researcher wants to target the structure to which RNA polymerase binds which structure would the researcher need to target A activator B operator

Which of the following characteristics gives nucleic acids such as DNA and RNA the ability to be used as the hereditary material for all living organisms Select all that apply A B C D DNA and RNA are molecules that are protected and stored in the nucleus of all living organisms DNA and RNA both contain a double helical structure which allows for large amounts of genetic information to be stored within a cell DNA and RNA can encode genetic information through the sequence of nucleotides along each strand DNA is a molecule that can replicate which allows Information to be passed down from

Read the scenario given below Recall that the GFP gene or green fluorescent protein gene is the gene that gives jellyfish the ability to glow A scientist observed that some jellyfish produce a brighter glow than other jellyfish The scientist conducts a study in which he compares the sequences of the GFP gene in 12 different jellyfish Two of the 12 jellyfish can produce a brighter glow than the other 10 Upon analyzing the results the scientist finds that the 2 jellyfish with the brighter glow contain a substitution mutation in the GFP gene which produces a slightly different protein The scientist expected to find that the 10 jellyfish with the same more dim glow all had the same sequence for the GFP gene However the scientist found that 1 out of the 10 Jellyfish contained a mutation that changed the genotype of the jellyfish but did not change the phenotype of the jellyfish since the jellyfish still had the same dim glow as the other 9 jellyfish Which of the following mutations results in no change in the phenotype of an organism despite a change in genotype A B silent mutation frameshift mutation

A sequence of nucleotides found in the DNA of house cats Felis catus codes for tail length A mutation in the gene can result in a shortened tail If a mutation is caused by inserting a nucleotide in a DNA sequence how will the mutation affect the DNA sequence or the re sulting protein A B C D The mutation will cause the reading frame of the DNA to shift resulting in different codons following the insertion mutation The protein will contain one changed amino acid but the rest of the amino acids in the protein will remain unchanged The DNA will contain one fewer nucleotide than the original DNA which will cause a change in one amino acid in the protein There would be no effect on the resulting protein due to the sequence being read in mount of three nucleotider

A scientist has extracted DNA from a specimen of an ancient rodent Which of the following techniques can be used to amplify the DNA of the rodent so that it can be analyzed A B C PCR DNA fingerprint DNA sequencing

Which of the following Identifies the mechanism by which a single transcribed DNA se quence can code for more than one polypeptide A transcriptional control B C alternative splicing translational control

10 With regard to enzymes why are vitamins necessary for good health Give examples DA

POSSIBLE P Which reaction represents the most accurate model of how matter is transformed and conserved in cellular respiration O CO2 H20 sunlight 02 C6H12O6 CO2 O C6H1206 6026CO2 6H2O ATP cell energy O C6H1206 02 CO2 H2O sunlight O 6CO2 6H2O sunlight 602 C6H1206

A Calculating bacterial transformation efficiency requires counting individual colonies and the more that is added the higher the transformation rate pepti doglycan B trans formation solution C DNA

Which statement is FALSE regarding individual colonies selected from the ampicillin amp laced plate A also grows on normal agar B does not have amp resistance C has the gene of interest D expresses the desired gene

Bacteria growing on a culture plate that exhibits lacks discriminate colonies and exhibits uncontrolled growth A a bacterial lawn B contamination C signs of transformation

To test the success of a bacterial transformation experiment one can wait and measure the excretions or use a n A tagged phage B an isotopic CaCl solution C ampR plasmid

If pGLO is used for bacterial transformation confirmation that transformants were produced comes from A R gene B pGLO C bacteria resistance D bacteria glow in UV light

Bacteria from a transformation experiment will produce transformants that ampicillin laced agar A will not grow on B can adapt to C consume

Which of the following is NOT a factor expected to affect bacterial transformation efficiency A restriction enzymes B plasma membrane C volume of virions D cell wall

Bacteria can recombine their DNA by transformation conjugation or A endosymbiosis B exocytosis C transduction D exponential growth

A common external vector for transferring DNA from one bacterium to another increasing genetic diversity is a A phage B moneran C pili

Bacteria with sex pili can reproduce which differs from the typical binary fission process of most bacteria A mitotically B sexually C frequently

The plasmid DNA that some bacteria carry can confer A a purple stain with sapphron B antibiotic resistance C a pink stain with sapphron antibiotic affinity

The R plasmid in some bacteria carry the gene for ampR which ampicillin A amplifies the needed dose of B stains purple on C confers resistance to

Which of the following types of diseases is most likely NOT caused by eubacteria A bubonic plague C syphillis B flu D anthrax

Western blotting Day 1 View the following video and answer the questions below What is the purpose of western blotting What is the role of the purple dye in electrophoresis What is the role or function of the negative charges in this assay Explain how relative protein amounts are evaluated and are useful for understanding protein biology

In biotechnology a structure like the one to the right a plasmid receives pieces of from other organisms and produces a desired product A enzymes B bacterial fragments C O DNA

Before insulin was bioengineered in the 1960 s insulin dependent diabetics used the insulin from A pigs B mice C angiosperms D hamsters

In gel electrophoresis a DNA sample moves through the agarose at rate dependent on its A hydrogen bond density B size and density C strength of charge D level of saturation

In gel electrophoresis a DNA sample is placed in a well in the agarose It moves across the gel to the end A negatively charged B positively charged C neutral I

In gel electrophoresis a DNA sample moves through and is separated into bands based on size and density A agarose B jello C mechanical separator I

The agarose used in gel electrophoresis is unique it has a specific porosity that separates molecules based on A color B charge C shape

A If you would like bacteria to produce insulin which of the following would you perform Insert a modified plasmid into a human B Insert desired gene into a plasmid such as the one pictured C Cut a piece of the plasmid and splice it with human DNA

To synthesize artificial insulin the insulin producing gene was isolated in humans and inserted into A mice B pigs C viruses

A common genetically modified organism GMO is corn which receives the gene for frost resistance A bacteria derived B lab syntesized C virus derived

can be used as genetic markers as they are unique to each individual much like a fingerprint B JU

Question 15 of 25 At which point does the splicing of mRNA play the largest role in regulating gene expression OA Before transcription OB During transcription OC During translation OD Before translation SUBMIT

Question 13 of 25 During translation which step occurs immediately after the ribosome reads an mRNA codon OA The ribosome attaches to the strand of mRNA OB The finished polypeptide is released OC The new strand of mRNA leaves the nucleus OD A tRNA molecule binds to the ribosome SUBMIT

Question 3 of 25 Which sentence is true about the allele frequencies in a population OA They always add up to 100 OB They can be determined by multiplying the total number of alleles by 100 OC They can be found by dividing the total number of alleles in the population by 100 OD They never add up to 100 SURMALIT

Extra Credit up to 5 pts When using bacteria to produce a particular protein why is it usually better to use the cDNA version of the gene rather than the genomic DNA version

From the beginning the English treated the Native Americans as O subjects of the Crown O separate nations O slaves O animals

Colonists evaded the Molasses Act of 1733 O through extensive smuggling O through legislation voiding the act O through the production of their own molasses O through the extensive use of refined sugar as a substitute

Which of the following are characteristics of the Incas cliff dwelling mound building irrigated agriculture desert dwellers