Biotechnology & its Applications Questions and Answers

Within DNA adenine will always bond with Spelling counts QUESTION 2 In DNA the 5 carbon sugar is and cytosine will always bond with while in RNA the sugar is

www witn Entrepreneurship in Biotech Bridge the gap fro classroom to commercializatic

8 How can a patient file a complaint regarding a DMEPOS item service a Call Medicare at 1 800 633 4227 b Call ACHC at 1 800 937 2242 c Call NABP at 1 847 391 4406 d A B

195 1 Who do you contact if you have any questions during your survey a Judee Olimpo and or DPM b DPM and or Field Evaluator c Judee Olimpo and or Field Evaluator d DPM and or Store Director

How does having both endocytosis and exocytosis transportation allow for advance functions in the Endomembrane can you name some examples of each process

In which of the following organelles would you expect to first find a protein destined to be secreted from a cell Lysosome Endosome Endoplasmic reticulum Golgi apparatus

Activation of the fatty acid converting it to fatty acyl SCOA requires the expenditure of 2 ATPs Use your answers from parts B and C to calculate the total number of ATPs generated from the metabolism of a saturated fatty acid with 22 carbon atoms including both the citric acid cycle and the B oxidation pathway as well as the initial ATP required to produce the acyl SCOA molecule that starts the process Express your answer as an integer View Available Hint s 152 Submit AEO Previous Answers ATP s

Using the techniques of genetic engineering you design a protein you want to accumulate within the ER of yeast cells To accomplish this goal you need to do nothing all proteins go through the ER incorporate radioactive amino acids into the protein incorporate the appropriate DNA sequence s to create signal sequences in the mature peptide incorporate appropriate mannose 6 phosphate groups

5 A pair of dominant alleles influence coat color in cattle wherein homozygotes for one allele have red coats homozygotes for the other allele have white coats and heterozygotes have roan coats that are produced by an intermix of both red and white hairs Suppose that you start with a herd of 20 red cows 40 roan cows 20 white cows one red bull two roan bulls and one white bull How you would manage your herd to achieve the following goals you can choose which bulls will mate with which cows a Maximize the number of roan calves produced Could you achieve 100 b Minimize the number of roan calves produced Could you achieve 0 c Produce only roan and red calves

How many grams of Potassium acetate MW 98 14g mol do I need to prepare a 225mM solution at a final volume of 250mL Don t add units to your answer Keep two decimal digits A

What is the purpose of engineering disulfide bonds What is the concern about extra disulfide bonds Why do we change Asn and Gln residue Explain the creation of De Novo Proteins OOOO000000000001000 O0100000000000000003 WOOD 0010000000000000000010000 0000000000000000001408 888 Minimal medium Give examples of biomaterials for medical use that improved by protein engineering

Cystic fibrosis is a Mendelian recessive allele The most common mutation associated with cystic fibrosis is the D F 508 mutation In the UK it is the most common cystic fibrosis variant accounting for 75 of cases However the worldwide frequency of cystic fibrosis associated mutations can vary greatly The frequency of the D F 508 mutation associated with cystic fibrosis in Romania was only 36 6 of cases Country United Kingdom Romania 9 408 9 996 AF 508 392 2 AF 508 AF 508 200 2 a Demonstrate the frequency of the D F 508 mutation is statistically significantly different

Mononucleosis is an infectious disease common in young adults A caused by the A Epstein Bar virus resulting in a lot of red blood cell deaths and danger of internal bleeding from trauma because of enlargement of the final lymph node called the A

Chromosome number and structure during G2 In a human somatic cell normal body cell that is in G2 what would be true about chromosome number and structure Check all that apply Check All That Apply These cells would be considered haploid n The chromosomes would be in the replicated state The chromosomes would be attached to microtubules at their centromeres These cells would contain homologous chromosomes

77 Polygamists believe that monogamy is not a natural state for human beings They feel that people should have multiple spouses at the same time rather than just one spouse Although polygamy is illegal in the United States there are many people around the world who engage in this practice including some who do so in the United States despite the legal restrictions Polygamists who live in the United States are an example of a subculture mainstream culture popular culture a b C counterculture

You will use this original strand of DNA to complete numbers 1 5 1 Write the mRNA sequence that would be made from the original strand of DNA circle the mRNA codons and write the amino acid sequence Original DNA Strand ITACAATAGACGGIAAACT mRNA Strand Amino Acid Sequence 2 Change the 7th nucleotide in the original DNA strand from a T to a C Original DNA Strand ITACAATAGACGGTAAACT Mutated DNA Strand mRNA Strand Amino Acid Sequence Specific Mutation Type 3 Add a G to the original DNA strand after the 4th nucleotide not replacing anything Original DNA Strand TTACAATAGACGGTAAACT Mutated DNA Strand mRNA Strand Amino Acid Sequence Specific Mutation Type 4 Change the 6th nucleotide in the original DNA strand from an A to a T Original DNA Strand TTACAATAGACGGTAAACT Mutated DNA Strand mRNA Strand

slopes downward 2 Discuss why the aggregate demand curve

Photosynthesis 1 Distinguish between autotrophic and heterotrophic nutrition 2 Distinguish between photoautotrophs and chemoautotrophs 3 Describe the structure of chloroplasts 4 Write a summary equation for photosynthesis 5 Explain the role of redox reactions in photosynthesis 6 Describe in general the two main stages of photosynthesis 7 Explain what happens when chlorophyll or accessory pigments absorb photons 8 List the components of a photosystem and explain their functions 9 Trace electron flow through photosystems II and I 10 Compare and contrast chemiosmosis in mitochondria which results in oxidative phosphorylation and photophosphorylation in chloroplasts 11 Describe the role of ATP and NADPH in the Calvin cycle 12 Summarize the Calvin cycle What goes in What comes out

10 Discuss the limitations of enacting fiscal policy during a recession

4 Explain why the short run aggregate supply curve is upward sloping

5 List the determinants of aggregate supply and explain how changes in these determinants affect the aggregate supply curve

What is recombinant DNA O plasmid human cell DNA nucleus insulin gene O recombinant DNA transgenic bacterium with plasmid containing insulin gene insulin Recombinant bacteria gain ability to secrete human insulin It is DNA that contains sequences from a single species It is DNA that contains sequences from two different species It is the process of replicating DNA segments using a bacterial enzyme It is the process of separating bacterial and human DNA strands using a gel

2 How do cells in different tissues with the same genome differ in terms of gene expression and what are the research applications related to these differences

Question 4 Which of the following regulatory elements are Cis acting DNA methylation O Transcription factors TATA box miRNA Euchromatin Question 5 20 pt 20 pi

What healthcare or community setting would you most prefer to enter as a Personal Support Worker Provide details What additional education would you like to engage in to enhance your Personal Support Worker capabilities and knowledge Provide details and reasoning Are you considering further post secondary education explain

If you have a series of PR sugar fermentation test tube results after proper incubation what would be the interpretation for each of the following observations Match the questions to the correct answers PR G is yellow and has a large bubble in the Durham tube PR L is red and has no bubbles in the Durham tube PR S is yellow and had no bubbles in the Durham tube Choose Choose Choose

Here is the formula to calculate bacterial concentration using the spread plate technique viable count CFU mL CFU x dilution factor over the volume of culture plated in mL What is the proper calculation of CFU mL in this solution series Sample 1 mL 1 mL 9 mL 1 10 10 1 1 mL TNTC Plaque counts Source wikisanitarc si 75 CFU x 10 4 per mL 1 mL 1 100 10 345 CELLY 10 4 per ml 9 mL din mL 1354 1 mL 9 mL 1 1000 10 3 1 mL 345 1 mL 9 mL 1 10 000 104 1 mL 75 9 mL 1 100 000 10 5 1 mL 10

In eukaryotes there are several different types of RNA polymerase Which type is involved in transcription of mRNA known as pre mRNA ligase RNA polymerase III RNA polymerase I RNA polymerase II

An area of skin that is innervated by a single cranial or spinal nerve is called a n O somite O dermatome O neuropathy receptive field

Sort the following enzymes according to whether they are used in glycolysis gluconeogenesis or both Drag each item to the appropriate bin View Available Hint s Glycolysis only pyruvate carboxylase pyruvate kinase hexokinase glucose 6 phosphatase triosephosphate isomerase Glycolysis and gluconeogenesis Gluconeogenesis only Reset

When a patient has Disorder the patient behaves in socially inappropriate ways that may include poor manners loss of decorum or rash impulsivity apathy or inertia reduced capacity for compassion compulsive behavior and hyperorality binge eating pica drinking smoking and alterations of diet

Can you please make a question about DNA repair in your own words

Can you please make a question about DNA repair in your own words

to reach your answer fraud Describe the method you used 4 Describe a way you could use DNA bar coding technology while researching the following a the types of fishes eaten by penguins in Antarctica 193 brauch lacky mond nie will dimpisq animistub eathory 212 gruscamos el rol 6 1slqarse hoolds of b whether the skins used for leather coats and purses came from endangered species stre Innodi Aptusy c contents of herbal supplements sold on the Internet or in health stores How is the information you could obtain by analysis of STR profiles different than ne information you could get from DNA bar coding Explain your answer

assay show silencer sequence in the regulator region of the BRCA2 gene in three different types of breast cancer cells MDA MB 231 breast cancer cells type 1 and BT549 cells breast epithelial ductal cancer cells type 2 and MDA MB 468 cells breast cancer cells type 3 PCR was performed with primers amplifying the silencer sequence and run on a gel and stained with ethidium bromide Silencing Complex Silencer SLUG Deacetylated Histones CBP 1 Promoter HDAC 1 Transcription Silencing Input SLUG 1 Based on the data which type of cancer cell has the repressor SLUG bound to the BRCA2 gene CIBP 1 2 Based on the data which type of cancer cell has the other members of the repressor complex bound to the BRCA2 gene HDAC 1 3 Based on the data which type of cancer cell in likely expressing the BRCA2 gene and which is not Why can you only predict a likely hood and not definitive expression Input 4 The input lanes are the results of the PCR using all of the sonicated DNA i e not precipitated with an antibody at all like shown in the schematic Step2 as the template in the PCR step of ChIP What is the role or purpose of the input data in this assay 5 Based on your understanding of cancer biology predeict what would the data look like sketch it if this assay was done on a normal breast epithelial cell Normal Breast epithelial cells 6 Based on the data is the BRACA gene an oncogene or TSG Explain

at is biomagnification

A cell lacks sister chromatids in a mitotically active tissue The cell in question is most likely in OS O GO G1

2n 18 How many chromosomes are present per cell at Prophase of Mitosis and are they replicated or unreplicated O9 unreplicated O 18 replicated 9 replicated 18 unreplicated

including labor and capital equilibrium in loanable funds market equilibrium in labor market balanced budget of the government perfectly flexible wages prices and interest rates Question 7 3 points tion of resources Listen What is NOT to blame for unemployment according to neoclassical economists labor union efficiency wage long term contract flexible wage

How does the herpes simplex virus genome reach the interior of an infected cell a The intact viral capsid travels down a scaffold of microtubules to a nuclear pore b The capsid proteins are disassembled upon entering the cytoplasm c The capsid remains outside the cell the nucleic acid is threaded through a receptor in the membrane O d The viral particle enters the cell by endocytosis and releases virion contents into the cytoplasm e DNA is forced into the capsid

Potential applications of bacterial oscillator switches include all the following EXCEPT a the study of controlled interactions between two feedback genetic loops b the design of bacterial genetic circuits to detect toxins c the design of bacterial clocks d reactivity to concentrations of specific molecules such as arabinose e the reduction of noise in biological circuits

Volume of Soil 8 8 2023 Level 1 5 cm 8 8 2023 Level 2 7 5 cm 8 8 2023 Level 3 12 cm 8 8 2023 Level 4 15 cm 3 10 2023 Level 1 5 cm 8 10 2023 Level 2 7 5 cm 8 10 2023 Level 3 12 cm 8 10 2023 Level 4 15 cm 8 16 2023 Level 1 5 cm 8 16 2023 Level 2 7 5 cm 8 16 2023 Level 3 12 cm 8 16 2023 Level 4 15 cm 8 20 2023 Level 1 5 cm 8 20 2023 Level 2 7 5 cm 8 20 2023 Level 3 12 cm 8 20 2023 Level 4 15 Leaves per branch 20 22 18 20 17 22 18 15 11 15 20 16 5 11 9 12

What properties of ES or ES like cells were measured in you assigned paper For each property of ES cells state what assays were used Question what does it mean with assays Does it mean experiments used or what procedures were used to measure or determine something what is an assay in biology exactly What properties FS may

Question 16 When a neurotransmitter released from one neuron binds to a O enzyme linked receptor O G protein coupled receptor nuclear receptor O ligand gated ion channel O voltage gated ion channel on a secondneuron that neuron depolarizes and fires

Following its initial activation how does a G protein become inactivated O The G protein a subunit GTP is hydrolyzed to GDP allowing reassociation of the G protein a subunit and By dimer The receptor becomes internalized O The G protein a subunit is digested and recycled O The G protein a subunit containing a GTP eventually reassociates with the G protein bg dimer O It spontaneously loses activity within a few seconds

Blue light has energy than red light and is by a green leaf more absorbed O less reflected more reflected O less absorbed

7 In guinea pigs black coat color is dominant over white coat color Show the genotypic and phenot ratios of the offspring of a mating between two heterozygous black guinea pigs 30 points SHOW YOUR WORK 1 What is the phenotypic ration of the offspring 3 1 2 What is the genotype ratios of the offspring 1 2 1

E coli Staphylococci spp Salmonella spp Fungi Question 6 Which of the following species would survive the otherwise sterilizing temperature of an autoclave 121 degrees Celsius O Psychrophiles 0

Which of the following genotype s would result in an individual with the type A blood phenotype Select all that apply ii Ai A A A B Question 14 0 5 pts

In fruit flies Drosophila an autosomal gene controls body color Individuals that are homozygous recessive have an ebony body color but are quite weak Individuals that are homozygous dominant have a normal body color and normal physical condition and heterozygous individuals have a normal body color with increased physical condition This is an example of epistasis Oheterozygote advantage polygenic inheritance multiple alleles