Lab Data K Volume 02 mL Time min Tube 1 Green Light Tube 2

What is the eventual fate of Glyceraldehyde 3 phosphate in the Calvin s cycle It fixes carbon dioxide to form 3 phosphoglycerate It is recycled to form Ribulose 1 5 bisphosphate RuBP a 5 carbon compound It is used to form sugars usually glucose by linking to 3 carbon G3P to form a six carbon sugar C6H12O6 forming the final product of photosynthesis Two of these are correct All of these are correct

6 What is the molecular weight of the large subunit of RuB A 14000 Daltons B 55000 Daltons C 37 kDa D 50 kDa 7 Which type of buffer is used during the elution step in ion exchange chromatography to displace proteins from the beads A High pH buffer B Low salt buffer C High salt buffer D Organic solvent 8 What is the purpose of heating samples with sample buffer before protein electrophoresis A To precipitate the proteins B To renature the proteins C To denature the proteins and reduce disulfide bonds D To sterilize the samples True False Questions 1 RuBisCO is an efficient enzyme with high CO2 fixation rates True False Secondary structure of proteins involves hydrogen bonding between polypeptide chains True False 2 In ion exchange chromatography the stationary phase is typically nonpolar True False 3 Proteins can be separated based on their solubility differences using ammonium sulfate precipitation True False Calculation Questions 1 If you have a protein solution of 100 mL how much ammonium sulfate in gram is needed to reach a saturation point of 37 given that the required amount is 210 g L 2 For a 50 saturation of ammonium sulfate how many grams would be neede per liter

What would you expect to happen if you compare the rate of photosynthesis in bright white light and in darkness Multiple Choice O The rate of photosynthesis will not be affected by bright white light or darkness The rate of photosynthesis will be less under bright white light than in darkness The rate of photosynthesis will start slowly under bright white light and then increase in darkness The rate of photosynthesis will remain constant in bright white light and darkness The rate of photosynthesis will be greater in bright white light than in darkness

In paper chromatography photosynthetic pigments from a plant pigment extract will separate due to their Check all that apply Check All That Apply differences in molecular weight differences in solubility in a chromatography transport solution mobile phase differences in affinity for the chromatography paper stationary phase differences in boiling point differences in pH

Multiple Choice O the season of the year O the color of light the color of the leaf the color of light that it reflects the color of light that it absorbs

Different photosynthetic pigments from a plant pigment extract can be separated by Multiple Choice O a respirometer O a capillary tube paper chromatography a barometer

Chlorophyll is the main pigment found in plants Other pigments are also present If these other pigments were lost from a plant but chlorophy remained what effect would this have on the plant Multiple Choice The plant would no longer be able to absorb photons The plant would no longer be able to make NADPH O O The plant would no longer be able to absorb wavelengths of light distinct from the ones absorbed by chlorophyll The plant would no longer be able to make ATP The plant would no longer be able to fix carbon

Match each pigment with its color 1 carotene 4 chlorophyll b 2 chlorophyll a 3 xanthophyll Match each of the options above to the items below yellow orange yellow blue green yellow green

How does a phenol red containing solution look if CO2 level is low Multiple Choice green red O yellow O pink colorless

The primary photosynthetic pigment is Multiple Choice chlorophyll phaeophytin xanthophyll phycobilin