The structure of enzymes that just includes folding AND

What does real time mean in rRT PCR A relatively small amount of PCR product can be quantified All of these choices It can be used to screen for results in Real Time We can monitor the progress of a PCR while its running

Describe the genetic material of SARS CoV 2 O ds DNA ve sense ssRNA ve sense ssRNA 5 DNA Question 4 1 point Listen Why do we use no template control in the reaction To screen for CDNA To test for viral RNA To screen for any contamination in the reagents

Directions After identifying the amino acid sequences for your gene follow the model below to construct the resulting structure of each protein on your own sheet 1 Using graph paper provided start your sequence in the third box down and third box from the left 2 The original protein will be contained within the first sheet and the other three proteins should each be on their own sheets for comparison 3 Write the first three letters of the amino acids into the three boxes if space allows Where boxes may be too small shade the boxes in and label each amino acid next to that shape 4 Use the key on the following page to assemble each protein Once all 4 models have been drawn on your graph paper you can more easily determine where the shape of the protein has changed if at all Circle the point at which the shape first changed in each altered protein Answer the following questions based on your completed translated sequences What is an addition insertion What is a substitution What is a frameshift What is a silent mutation Of the four mutation types above which can cause one of the others Explain using evidence

DNA mRNA OR 1 2 4 5 1 3 4 5 Ligand A Ligand B Protein Protein A Plasma Membrane Protein B Plasma Membrane Exon Intron 2nd Messenger Figure 1 Receptor proteins produced as a result of alternative splicing followed by activation of a second messenger 4 Cell signaling in eukaryotes is often dependent on receptor proteins located in the plasma membrane During the production of the mature mRNA molecules coding for these receptors pre mRNA molecules are processed to remove introns and to connect exons together The exons contain the sequences that code for proteins In certain instances different mature mRNA molecules can be formed from the same pre mRNA by alternative splicing which results in different protein sequences in the resulting polypeptides Figure 1 represents the expression of a gene with 5 exons that can be alternatively spliced to produce receptor protein A and receptor protein B a Explain how ligand A and ligand B can cause identical cellular responses in a cell b Predict the most likely effect of a two nucleotide deletion in the middle of the intron located between exc 4 and 5 on the structure of protein A Justify your prediction

Variations on Mendelian Inheritance Two gene cross blue eyes Select the crosses that would lead to only blue eyed progeny Choose all that apply Check All That Apply oohh x oohh

Green Genotypic ratio of offspring AA Aa aa 4 If you had a plant that developed with a purple stem what type of plant could you cross it to tell if it were homozygous or heterozygous Explain using a Punnett square

B galactosidase is encoded by the of the lac operon QUESTION 2 extends from the termination codon at the end of an mRNA coding region to the end of the poly A tail QUESTION 3 In prokaryotes nearly all the DNA encodes RNAS or proteins with little between individual transcription units

Melosis 1 MEIOSIS Independent assortment Independent assortment takes place during Metaphase I This is when homologous chromosomes line up in the middle of the cell in a random orientation This results in different combinations of chromosomes in each daughter cell KEY 88 XX XX XX Drag drop the chromosomes from the parent cells into the daughter cells to show how the possible combinations of chromosomes Maternal chromosome came from the mother 88 s OO Paternal chromosome come from the father

QUESTION 24 What was the new name of Beadle and Tatum s hypothesis after it was discovered that some enzymes were composed of more than one polypeptide chain O the One Gene One Enzyme hypothesis the One Gene One Polypeptide hypothesis O the One Polypeptide One Enzyme hypothesis O the One Polypeptide One Gene hypothesis O the One Gene Two Gene hypothesis QUESTION 25 What are the three distinct activities of protein synthesis in the correct order O initiation elongation termination O termination initiation elongation O initiation termination elongation O elongation initiation termination

b How many copies of the Wallele are present in the gene pool What is the frequency of the Wallele Show your work 3 points aloon snobne natteliv siterap of colalls at nonclugod mill chep dose not How many copies of the B allele are present in the gene pool What is the frequency of the B allele Show your work 3 points