1 Gather PCR tubes for your samples allocating two PCR tubes

Last updated: 8/27/2023

1 Gather PCR tubes for your samples allocating two PCR tubes for each one one set of tubes will be for the presence of 35S and the other set will be for the presence of tubulin You will have six tubes in total 2 Set up the PCR tubes for 35S a Obtain one PCR tube containing Ready To Go PCR beads for each sample three in total and label with the sample type group color and 35S b Add 22 5 L of the 35S primer loading dye mix to each tube Allow several min for the beads to dissolve c Add 2 5 L of each DNA sample to its respective tube