Biology Topics

D Question 3 transcript Identify this phase of meiosis 4 D

B Question 2 Itranscript Describe these structures O more than one a tetrad 4 B

O Anaphase Il O Anaphase I O Metaphase II

1 Convert a 5 mL to L b 650 L to mL c 40 L to mL d 10 mL to L e 1500 mL to L 2 What micropipettor p2 p20 p200 or p1000 would you use to pipet the amounts above ar would the window read example to pipette 250 L I would use the p1000 set at 025 If the volum too large for a single pipettor how would you work around this

You counted 5 cells on one large grid of the hemocytometer The sample was diluted 1 1 in trypan blue What would be your final cell ml concentration of the cell sample O 5 x 10 cells ml O 2 x 10 cells ml O 2x 105 cells ml 5 X 104 cells ml

To genetically transform an entire organism you must insert the new gene into every cell in the organism Which organism is better suited for total genetic transformation one composed of many cells or one composed of a single cell Explain

All the following are true statements regarding compound light microscopes except Closing and opening of the iris diaphragm can increase or decrease the contrast of the observed specimen Viewing a sample with the 10X objective would magnify the specimen 1000 times its actual size Magnification in which a specimen is being viewed can be changed by use of different objective lenses O The condenser focuses the light source on a specimen

Trypsin is a pH indicator often added to cell media True O False

You performed a bacterial transformation a What did you transform and how b How do you select for bacteria that contain your DNA of interest

What are two uses for cell culture

1M sodium pyrophosphate is required for skeletal muscle contraction O True O False

Label the 5 reagents necessary for a successful Polymerase Chain Reaction and their function

DNA has been loaded onto an agarose gel a Does DNA carry an overall positive neutral or negative charge b When placed in the gel box DNA migrates toward which electrode c Does DNA fragment size have an effect on how fast DNA migrates through a gel Explain d Why are we able to see DNA fragments in the gel when it is exposed to UV light

What are the three steps to a cycle of a PCR reaction and what occurs in these steps

All the following are types of substrates used in mammalian cell culture to potentially increase cell adhesion except O Tissue culture O Collagen Poly L lysine Phenol red

Describe 5 key techniques you utilized to reduce the risk of contamination of your mammalian culture cells