1 Gather the gel electrophoresis apparatus including the gel tray and power supply Make sure the gaskets are secured within the

Question

1 Gather the gel electrophoresis apparatus including the gel tray and power supply Make sure the gaskets are secured within the grooves found at each end of the gel tray and place perpendicular into the gel electrophoresis box 2 Prepare 60 mL 1 5 agarose gel in 1x TBE in an Erlenmeyer flash Heat in the microwave at 30 sec intervals until boiling and the agarose is completely dissolved 3 When the solution is cool enough to touch comfortably add GelStar 1 L GelStar per 10 mL of agarose solution Pour the mixture into the gel tray and insert the well comb 4 When gel has solidified remove the comb and align gel tray to be parallel with the gel box making sure that the wells are closest to the black electrodes Fill the gel cast with 1x TBE until the buffer completely covers the gel 5 Ask your TA to load 3 L of GeneRuler 1kb Plus DNA digest Ladder onto the first lane 6 Load the entirety of each sample 25 L onto the gel BE SURE TO WRITE DOWN THE ORDER IN WHICH YOU LOADED YOUR SAMPLES in your notebook 7 Run the gel at 70V for approximately 20 30 minutes and then increase to 90V for 30 40 minutes or until bands have migrated down three fourths of the gel 8 Remove gel from between the caster carefully to a weight boat with TBE from the gel apparatus Give your gel to your TA to transfer the gel to the UVP analyzer to view under UV light DO NOT LOOK AT THE UV LIGHT Print one image of the gel under UV light and save the image to a USB flash drive

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