1 Measure the distances of the bands of the MW ladder from

Last updated: 8/27/2023

1 Measure the distances of the bands of the MW ladder from the wells to the middle of the bands 2 Plot a standard curve by distance traveled through the gel versus log bp and use the equation that determines how distance traveled y varies with log bp x 3 Measure the distances of the bands produced by each restriction enzyme experiment 4 Use the standard curve equation to determine the size of the fragments for each lane This is achieved by submitting the migration distance of your samples as the y variable in the equation you generated in step 2 Solving for x provides the MW of your samples GeneRuler 1 kb Plus DNA Ladder ready to use bp 20000 10000 7000 5000 4000 3000 2000 1500 1000 700 500 400 75