2 Refer to the lac operon diagram below The plus and minus symbols in the Glucose and Lactose columns indicate the presence and

Question

2 Refer to the lac operon diagram below The plus and minus symbols in the Glucose and Lactose columns indicate the presence and absence of each sugar in the media glucose lactose CAP site CAP site CAP site CAP site P P 0 lacZYA lacZYA lacZYA lacZYA lacZYA transcription a Under which conditions state whether glucose and lactose are present or absent would Lac repressor bind to DNA Where on the DNA would it bind b Under which conditions state whether glucose and lactose are present or absent would CAMP CAP bind to DNA Where on the DNA would it bind c Which combination s of Lac repressor and CAMP CAP binding or not binding will allow the strongest lacZYA expression Where would RNA Polymerase bind to the DNA for the strongest lac lacZYA expression d Now think about E coli inoculated into fresh media containing both glucose and lactose at time 0 Which of the conditions state whether glucose and lactose are present or absent in the diagram above would be encountered by those E coli and in which order Justify your answer briefly

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