A scientist wants to study the kinetics of a specific membrane protein. They ligate the DNA coding region of the protein to the

Question

A scientist wants to study the kinetics of a specific membrane protein. They ligate the DNA
coding region of the protein to the DNA coding region for GFP and introduce the DNA into
cells. A day or two later, they observe the cells under the microscope and they are
fluorescing green. They then shine a laser at a region of the cell repeatedly (or
continuously) that region loses its fluorescence. They monitor a separate region of the
cell and find that fluorescence never decreases in that area. What is the full name of the
technique that they performed? What can they conclude from their result?
a) What is the full name of the technique that they performed?
The technique is known as Fluorescence Loss in Photobleaching (FLIP).
b) What can they conclude from their result?

Kunduz · Accepted Answer

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