A student assays a newly purchased enzyme by the standard procedure. He dissolves 50mg of the enzyme in 100 mL water and uses

Question

A student assays a newly purchased enzyme by the standard procedure. He dissolves 50mg of the enzyme in 100 mL water and uses 10mL for the assay. He adds the enzyme to the substrate and collects absorbance data at specified intervals. Calculate the specific activity of the enzyme if at 5 minutes absorbance was 0.345 and at 10 min absorbance was 0.645. Standard curve equation was given as Y=0.238x. Show work to get full points

Kunduz · Accepted Answer

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