A student placed a sample of a food coloring that contains a

Last updated: 7/10/2022

A student placed a sample of a food coloring that contains a mixture of a blue dye and a red dye at the top of a chromatography column filled with a nonpolar stationary phase. When water is poured through the column, two bands of colors are seen in the column, as shown in the diagram above. A student collects a sample of the blue dye from the column in a cuvette. Which of the following is least useful to determine the concentration of the sample using a spectrophotometer set at 640 nm? The volume of the sample The path length of the cuvette The absorbance of the dye at 640 nm The molar absorptivity of the dye at 640 pm