Any permanent alteration of the genotype of an organism is referred to as transformation For example if strain of brewer s yeast

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Any permanent alteration of the genotype of an organism is referred to as transformation For example if strain of brewer s yeast Saccharomyces cerevisiae lacks the genes necessary to synthesize the amino acic histidine abbreviation His it is unable to grow on medium without histidine One can transform cells of this Recipient strain using DNA from a wild type Donor strain to restore the ability to make histidine Shown below are partial results of such an experiment Minus signs indicate no growth and plus signs indicate growth What can you reasonably conclude from these results Sample Plate Donor cells Recipient cells no transformation Transformed Recipient cells His His The Donor cells were contaminated with Recipient cells so the results are inconclusive O This experiment was totally successful as all of the controls gave the expected result The Recipient cells were contaminated with Donor cells so the results are inconclusive The Recipient cells were contaminated with DNA so the results are inconclusive 4

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