AVY Site Specific Methylation 100 75 50 25 0 Control Diet

Last updated: 8/19/2023

AVY Site Specific Methylation 100 75 50 25 0 Control Diet BITT 1 2 3 CG site number I BPA Diet TITT CG site number p 0 05 p 0 01 The viable yellow allele has provided a valuable tool for investigating the impact of diet on epigenetic regulation of obesity since the Agouti gene also has a poorly understood role in body weight regulation A study published in 2007 Dolinoy et al Proc Nat Acad Sci USA 104 13056 13061 investigated effects of BPA bisphenol A a chemical used in the manufacturing of plastics which is associated with elevated body weight and other adverse health outcomes in humans on methylation of the IAP insertion in the viable yellow allele The figure above shows that mice with the viable yellow allele whose mothers were fed a BPA containing diet during pregnancy had significantly lower levels of methylation at multiple CG dinucleotides within IAP compared to mice whose mothers were fed a control diet What difference in fur color would you expect to see between mice in the two groups above mice whose mothers were fed control diet vs BPA diet Provide one sentence of rationale for your answer based on what you know about the impact of CG methylation on gene expression including a clear prediction regarding the expression level of the Agouti gene in the two groups