Components O Brown tube Phusion HF buffer Blue tube TPS

Last updated: 11/2/2023

Components O Brown tube Phusion HF buffer Blue tube TPS Green tube RNA forward Pink tube RNA reverse Yellow tube A template Clear tube usion DNA polymerase tal volume of the stock sample 5X 10 mM 10 M 10 M 0 05ng L 2 U L concentration in reaction tube 1X 0 2 mM 0 2 M 0 2 M 0 5ng 100 L 1U 100 L per reaction Positive control L 63 5 L 20 0 L 2 0 L 2 0 L 2 0 L 10 0 L 0 5 L 100 L per reaction Negative control L 73 5 L 20 0 L 2 0 L 2 0 L 2 0 L 0 5 L 100 L