FRET was used to study the interaction between 2 molecules.

Last updated: 8/8/2022

FRET was used to study the interaction between 2 molecules. Fluorophores used were Cyan Fluorescent Protein (CFP) and Yellow Fluorescent Protein (YFP). CFP absorbs 430nm light and emits 500nm light. YFP absorbs 500nm light and emits 580nm light. The molecule onto which CFP binds to, can act as Both as fluorescent donor and acceptor It does not give off any fluorescence Fluorescent donor Fluorescent acceptor