How can one experimentally determine how many solvent accessible thiol groups there are per molecule of BSA Bovine Serum Albumin

Question

How can one experimentally determine how many solvent accessible thiol groups there are per molecule of BSA Bovine Serum Albumin from a BSA sample of unknown concentration What would be the experimental design detailed with related calculations Ellman s reagent and related equipment spectrophotometer buffers etc are at your disposal Thanks in advance please let me know if any more information is needed and I will be sure to reply

Kunduz · Accepted Answer

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