Imagine that you repeat Seymor Benzer s tRNA Selection

Last updated: 5/13/2023

Imagine that you repeat Seymor Benzer s tRNA Selection experiment with modifications as follows 1 Synthesize mRNA containing A s and G s only poly AG in random order 2 Convert the amino acid Glutamic acid Glu on its tRNA to the amino acid Glutamine Gln as shown below 3 Mix your poly AG RNA your artificial tRNA and cell extract contains ribosomes amino acids all normal tRNAs and the energy source for translation Glu Gin CUC CUC Glutamic acid Glu is encoded by GAA and GAG while Glutamine Gln is encoded only by CAA and CAG Will Glutamic acid Glu be found in the resulting polypeptides this is a bit tricky so review the contents of the assay above Yes because ribosomes select the amino acid regardless of the anticodon sequence Yes because all of the normal tRNAs are present in the cell extract so Glutamic acid can also inserted when GAG or GAA codons appear in the mRNA Yes for both of the reasons listed above No because only Gin is present on the tRNA with the CUC anticodon