In the protein isolation experiment what did we do to make the negatively charged proteins pass through the positively charged

Question

In the protein isolation experiment what did we do to make the negatively charged proteins pass through the positively charged beads We used increases and decreases in temperature to build amplicons We used partial pressures to drive samples through the beads We used G forces to push the samples through the beads We used salts of increasing concentrations to release proteins from the beads

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