Learning O Macmil You are a technician in a biochemistry lab running receptor binding experiments The target membrane bound

Question

Learning O Macmil You are a technician in a biochemistry lab running receptor binding experiments The target membrane bound receptor has been partially purified from mouse rat and human cell lines Using the same radioactive ligand in a saturation binding assay for each species receptor you generate the binding data in the table The dependent variable Y is the fraction of binding sites occupied by the ligand Ligand concentration nM 0 20 0 50 1 0 4 0 10 20 50 human receptor K rat human Y for mouse receptor 0 048 0 11 mouse 0 20 0 50 0 71 0 83 0 93 Y for rat Y for human receptor receptor Determine the K for the human receptor in this binding experiment Which receptor binds most tightly to this ligand 0 29 0 50 0 67 0 89 0 95 0 97 0 99 0 17 0 33 0 50 0 80 0 91 0 95 0 98 nM

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