MARION M BRADFORD 0 5 0 4 03 Absorbance at 595nm 000 01210

Last updated: 9/4/2023

MARION M BRADFORD 0 5 0 4 03 Absorbance at 595nm 000 01210 30 50 70 Time in Minutes complex formation rate and color stability FIG 3 Protein dye mples during one of the flatter portions of the color stability curve be ween 5 and 20 min after reagent addition This still gives ample time to ad a relatively large number of samples Microassay system sensitivity When bovine serum albumin is used as me standard in the micro assay system the degree of nonlinearity is milar to that found in the standard assay There is a loss in protein dye omplex response as compared with the standard assay i e 5 g rotein ml gives an absorbance change of 0 1 vs 0 27 in the standard as ay Perhaps this results from increased dilution of the protein reagent Interference by nonprotein components As indicated earlier there is some interference in the assay system by strongly alkaline buffering agents This may be overcome by running the appropriate buffer con rols and subtracting the value for the control either mathematically of spectrophotometrically A wide spectrum of components was tested for effects on the protein dye binding assay Table 1 A lack of effect on the assay by magnesium chloride potassium chloride sodium chloride ethanol and ammonium sulfate was observed The small effects due to Tris acetic acid 2 mercaptoethanol sucrose glycerol EDTA and trace quantities of the detergents Triton X 100 sodium dodecyl sulfate and C In the pre lab video instructions stated to incubate Bradford dye protein for 10 minu According to Figure 3 in the paper how much would the absorbance change if I waited hour to make my measurement