Master mix 1 GFP pUC19 F 5 CCAGGCTTTACACTTTATGCTTCC 3 GFP

Last updated: 2/7/2024

Master mix 1 GFP pUC19 F 5 CCAGGCTTTACACTTTATGCTTCC 3 GFP PUC19 R 5 CTTTGATTCCATTCTTTTGTTTGTCTGCC 3 Master mix 2 GFP PBR322 F 5 GATGACGATGAGCGCATTGTTA 3 GFP PBR322 R 5 CTTTGATTCCATTCTTTTGTTTGTCTGCC 3 Reagents Sterile water Taq buffer dNTPs Forward primer Reverse primer Bacterial DNA Taq polymerase Stock concentration 10X 2mM 2 M 2 M Final concentration 1X 0 2mM 0 2 M 0 2 M Final volume Volume L 1 reaction 2 L 1 L 20 L 7 reactions 126 L Question Fill in the required volumes for the PCR table above 6 Place your labelled PCR tubes in the PCR machine closest to you Once you and the group opposite you have loaded your tubes into the machine program the following conditions