What is the purpose of having the three positive controls in the PCR experiment? In order to determine the genotype of the

Question

What is the purpose of having the three positive controls in the PCR experiment?
In order to determine the genotype of the positive controls.
To make sure enough cheek cells were extracted at the beginning of the lab.
For comparison in order to determine the genotype of the unknown sample.
To be able to make sure the gel ran in the correct direction.

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