You are working on the trp operon in a lab and are working with two mutants In Mutant A the operator region of the operon

Question

You are working on the trp operon in a lab and are working with two mutants In Mutant A the operator region of the operon carries a mutation that decreases affinity of the repressor for the operon Mutant B expresses a trp repressor protein that cannot bind tryptophan but still has good affinity for the operator a super repressor Determine whether the following would be true in Mutant A Mutant B neither or both These mutants will show a wildtype normal phenotype at all tryptophan concentrations if a non mutated trp repressor could be inserted in the genome and expressed from it These cells die if tryptophan is omitted from their medium due to lack of tryptophan availability In the absence of tryptophan RNA polymerase holoenzyme can access the promoter of the trp operon in these cells In these cells tryptophan binds directly to the DNA regulating RNA polymerase Choose Choose Choose Choose

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