In 1944 Avery, MacLeod, and McCarty performed transformation

Last updated: 7/9/2022

In 1944 Avery, MacLeod, and McCarty performed transformation experiments using live, harmless bacteria and extracts from virulent bacteria treated with various enzymes. Which of the following enzymes were used and why? (A) Proteases and RNases to rule out protein and RNA as the transforming factors (B) Lipase (an enzyme that facilitates the breakdown of lipids) to rule out lipoproteins as the transforming factor (C) Kinase (an enzyme that facilitates transfer of a phosphate group from ATP to a substrate molecule) to show that transformation is phosphorylation dependent (D) ATPase to show that transformation is not dependent on ATP