Question:
A researcher synthesizes a new molecule called inhibitor X
Last updated: 2/25/2023
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A researcher synthesizes a new molecule called inhibitor X that inhibits the activity of phosphofructokinase PFK the enzyme that phosphorylates fructose 6 phosphate during glycolysis Inhibitor X competes with ATP for binding to the PFK active site The researcher determines that the Michaelis Menten constant Km with respect to ATP is 40 M When 2 M inhibitor X is added to the purified PFK reaction the apparent Km for ATP increases to 48 M Calculate the dissociation constant of the PFK inhibitor complex K