FRET was used to study the interaction between 2 molecules

Last updated: 7/10/2023

FRET was used to study the interaction between 2 molecules Fluorophores used were Cyan Fluorescent Protein CFP and Yellow Fluorescent Protein YFP CFP absorbs 430nm light and emits 500nm light YFP absorbs 500nm light and emits 580nm light The molecule onto which CFP binds to can act as O Fluorescent donor O Both as fluorescent donor and acceptor O Fluorescent acceptor It does not give off any fluorescence