Biotechnology & its Applications Questions and Answers

Biologists think that endosymbiosis gave rise to mitochondria before plastids partly because O the products of photosynthesis could not be metabolized without mitochondrial enzymes mitochondrial DNA is less similar to prokaryotic DNA than is plastid DNA without mitochondrial CO2 production photosynthesis could not occur all eukaryotes have mitochondria or their remnants whereas many eukaryotes do not have plastids
Biology
Biotechnology & its Applications
Biologists think that endosymbiosis gave rise to mitochondria before plastids partly because O the products of photosynthesis could not be metabolized without mitochondrial enzymes mitochondrial DNA is less similar to prokaryotic DNA than is plastid DNA without mitochondrial CO2 production photosynthesis could not occur all eukaryotes have mitochondria or their remnants whereas many eukaryotes do not have plastids
List the causes of the Great Depression Describe Hoovervilles including why they existed and who lived in them How did President Roosevelt respond to the Supreme Court declaring New Deal Programs Agricultural Adjustment Act unconstitutional
Biology
Biotechnology & its Applications
List the causes of the Great Depression Describe Hoovervilles including why they existed and who lived in them How did President Roosevelt respond to the Supreme Court declaring New Deal Programs Agricultural Adjustment Act unconstitutional
uestion 5 Analagous structures are anatomical structures in organisms that share the same basic form O True O False
Biology
Biotechnology & its Applications
uestion 5 Analagous structures are anatomical structures in organisms that share the same basic form O True O False
Which of the following bonds contains the most amount of potential energy C C O C C H Q H
Biology
Biotechnology & its Applications
Which of the following bonds contains the most amount of potential energy C C O C C H Q H
What is an average concentration of bacterial and archaeal cells in marine water at several thousand meters depth O 10 cells ml O 108 cells ml O 106 cells ml O 10 cells ml
Biology
Biotechnology & its Applications
What is an average concentration of bacterial and archaeal cells in marine water at several thousand meters depth O 10 cells ml O 108 cells ml O 106 cells ml O 10 cells ml
O Extension O Annealing O Denaturation O Translation Question 10 How does DNA travel from negative to positive By car From cathode to anode From anode to cathode
Biology
Biotechnology & its Applications
O Extension O Annealing O Denaturation O Translation Question 10 How does DNA travel from negative to positive By car From cathode to anode From anode to cathode
Which of the following tests is not required as part of the donor processing procedure for allogeneic donation O ABO O Anti CMV O Anti HTLV I O Rh O Cross match
Biology
Biotechnology & its Applications
Which of the following tests is not required as part of the donor processing procedure for allogeneic donation O ABO O Anti CMV O Anti HTLV I O Rh O Cross match
Which is not an example of the most common form of error associated with fatal transfusion reactions O Wrong RBC unit is tagged for transfusion O Miss identification of patients at the time of transfusion O Antibody below detectable levels during pretransfusion testing O Phlebotomist labels patient A tubes with patient B information O Technologist enters results of patient A testing into patient B field
Biology
Biotechnology & its Applications
Which is not an example of the most common form of error associated with fatal transfusion reactions O Wrong RBC unit is tagged for transfusion O Miss identification of patients at the time of transfusion O Antibody below detectable levels during pretransfusion testing O Phlebotomist labels patient A tubes with patient B information O Technologist enters results of patient A testing into patient B field
Which of the following does NOT apply to lipids O A help to maintain body temperature B provide a readily available energy source preferred by cells O C form essential structural components of cells inst shocks
Biology
Biotechnology & its Applications
Which of the following does NOT apply to lipids O A help to maintain body temperature B provide a readily available energy source preferred by cells O C form essential structural components of cells inst shocks
WA 6 Replication Crisis Write an essay on some aspect of the replication crisis Or write about a claim once widely accepted that is now disputed discredited or doubtful Cite your source
Biology
Biotechnology & its Applications
WA 6 Replication Crisis Write an essay on some aspect of the replication crisis Or write about a claim once widely accepted that is now disputed discredited or doubtful Cite your source
Exercise 2 Questions Question 1 Describe the enzyme required to complete the recombinant DNA plasmid initiated by this exercise and the resulting size of molecule that would be produced Reference Data Table 1 in your answer BI U T T 0 Word s
Biology
Biotechnology & its Applications
Exercise 2 Questions Question 1 Describe the enzyme required to complete the recombinant DNA plasmid initiated by this exercise and the resulting size of molecule that would be produced Reference Data Table 1 in your answer BI U T T 0 Word s
Quorum sensing involves the production and monitoring of signaling molecules AND is used by eukaryotes to sense the presence of bacteriawithin their population is used by bacteria to limit the density of cells in a population AND is used by eukaryotes to sense the presence of pathogens within their cells O is used by bacteria to limit the density of cells in a population AND is used by eukaryotes to sense the presence of bacteriawithin their environment involves the production and monitoring of signaling molecules AND is used by bacteria to sense the density of cells within their population is used by bacteria to sense the density of cellswithin their population AND is used by eukaryotes to sense the presence of bacteria in their environment
Biology
Biotechnology & its Applications
Quorum sensing involves the production and monitoring of signaling molecules AND is used by eukaryotes to sense the presence of bacteriawithin their population is used by bacteria to limit the density of cells in a population AND is used by eukaryotes to sense the presence of pathogens within their cells O is used by bacteria to limit the density of cells in a population AND is used by eukaryotes to sense the presence of bacteriawithin their environment involves the production and monitoring of signaling molecules AND is used by bacteria to sense the density of cells within their population is used by bacteria to sense the density of cellswithin their population AND is used by eukaryotes to sense the presence of bacteria in their environment
Which one of the following things is NOT performed by proteins Select one O a defend against microorganisms b hormonal function c transport of substances O d catalysis e storage of genetic information
Biology
Biotechnology & its Applications
Which one of the following things is NOT performed by proteins Select one O a defend against microorganisms b hormonal function c transport of substances O d catalysis e storage of genetic information
PRE LAB QUESTIONS Read through the lab introduction and procedures and answer the following questions a How does the movement of molecules compare between solutions at room temperature versus 45 degrees Celsius b Which solution would have a faster rate of diffusion What is osmosis C d This movement or diffusion of water across a membrane is called e What is a concentration gradient f What is meant by a steeper concentration gradient g Elodea is a plant that lives in fresh water ponds The pond water has a lower concentration of dissolved particles than inside of a cell so fresh water will tend to move into the cell Is freshwater hypotonic isotonic or hypertonic compared to the Elodea cells h You can tell that the water fills the whole cell because the green chloroplasts are free to spread everywhere even out to the edges against the cell wall Would a cell in fresh water be considered lysed turgid crenated or plasmolyzed Explain PART 1 OSMOSIS AND TEMPERATURE 3 Hypothesis regarding the relationship of the rate of osmosis and temperature
Biology
Biotechnology & its Applications
PRE LAB QUESTIONS Read through the lab introduction and procedures and answer the following questions a How does the movement of molecules compare between solutions at room temperature versus 45 degrees Celsius b Which solution would have a faster rate of diffusion What is osmosis C d This movement or diffusion of water across a membrane is called e What is a concentration gradient f What is meant by a steeper concentration gradient g Elodea is a plant that lives in fresh water ponds The pond water has a lower concentration of dissolved particles than inside of a cell so fresh water will tend to move into the cell Is freshwater hypotonic isotonic or hypertonic compared to the Elodea cells h You can tell that the water fills the whole cell because the green chloroplasts are free to spread everywhere even out to the edges against the cell wall Would a cell in fresh water be considered lysed turgid crenated or plasmolyzed Explain PART 1 OSMOSIS AND TEMPERATURE 3 Hypothesis regarding the relationship of the rate of osmosis and temperature
b Why do you flame the mouth of the tube and heat the loop to red hot c What would your results have looked like if you forgot to sterilize the loop after inoculating the Serratia and before gathering the sample from Kokuria d What do you think are the benefits to storing bacteria on a slant versus a flat agar tube Discuss the biology of the two species of bacteria we worked with in this part of the lab Where are they found and what role if any do they play in human health and disease
Biology
Biotechnology & its Applications
b Why do you flame the mouth of the tube and heat the loop to red hot c What would your results have looked like if you forgot to sterilize the loop after inoculating the Serratia and before gathering the sample from Kokuria d What do you think are the benefits to storing bacteria on a slant versus a flat agar tube Discuss the biology of the two species of bacteria we worked with in this part of the lab Where are they found and what role if any do they play in human health and disease
An amplicon is a PCR product and the DNA to be amplified is the template O True False
Biology
Biotechnology & its Applications
An amplicon is a PCR product and the DNA to be amplified is the template O True False
If a fluid has a low viscosity and suddenly releases a small amount of gas let s say CO2 will it explode Hint think of opening a can of soda pop OYes it will explode No it will not explode
Biology
Biotechnology & its Applications
If a fluid has a low viscosity and suddenly releases a small amount of gas let s say CO2 will it explode Hint think of opening a can of soda pop OYes it will explode No it will not explode
0 08cm 14 85mm 0 027m 62 m Give the result in m do not round up your answer write exact resu Your Answer Calculate
Biology
Biotechnology & its Applications
0 08cm 14 85mm 0 027m 62 m Give the result in m do not round up your answer write exact resu Your Answer Calculate
QUESTION 45 Cells grown in culture outside the body are called cells They have become an essential tool of cell and molecular biologists QUESTION 46 Drosophila melanogaster is considered as model organism for animal O True O False QUESTION 47 The rapidity and cost efficiency of DNA sequencing has made it possible to sequence virtually all of the genes present in the microbes of a given habitat This generates a collective genome for that habitat which has come to be called a n
Biology
Biotechnology & its Applications
QUESTION 45 Cells grown in culture outside the body are called cells They have become an essential tool of cell and molecular biologists QUESTION 46 Drosophila melanogaster is considered as model organism for animal O True O False QUESTION 47 The rapidity and cost efficiency of DNA sequencing has made it possible to sequence virtually all of the genes present in the microbes of a given habitat This generates a collective genome for that habitat which has come to be called a n
True or False Only enveloped viruses may have glycoproteins True False
Biology
Biotechnology & its Applications
True or False Only enveloped viruses may have glycoproteins True False
Activity Completed Question Value Which term is a tendency to prefer information that supports what a person thought in the first place confirmation bias belief perseverance the placebo effect informed consent to research
Biology
Biotechnology & its Applications
Activity Completed Question Value Which term is a tendency to prefer information that supports what a person thought in the first place confirmation bias belief perseverance the placebo effect informed consent to research
Multiple Choice O O O a semipermeable membrane an energy barrier a cell wall an impermeable membrane
Biology
Biotechnology & its Applications
Multiple Choice O O O a semipermeable membrane an energy barrier a cell wall an impermeable membrane
During osmosis water will move in which direction Multiple Choice O O O from higher temperature to lower temperature from the area with lower solute concentration to the area of higher solute concentration from a lower enery state to a higher energy state from the area with higher solute concentration to the area of lower solute concentration
Biology
Biotechnology & its Applications
During osmosis water will move in which direction Multiple Choice O O O from higher temperature to lower temperature from the area with lower solute concentration to the area of higher solute concentration from a lower enery state to a higher energy state from the area with higher solute concentration to the area of lower solute concentration
What prevents a plant cell from bursting in a hypotonic solution Multiple Choice O O a plant cell will not burst in a hypotonic solution because water is moving out of the cell O the cell wall O the presence of chloroplasts the plasma membrane the coll
Biology
Biotechnology & its Applications
What prevents a plant cell from bursting in a hypotonic solution Multiple Choice O O a plant cell will not burst in a hypotonic solution because water is moving out of the cell O the cell wall O the presence of chloroplasts the plasma membrane the coll
Multiple Choice O O The cell membrane did not change at all because of the protection of the cell wall The cells burst because the solution was hypotonic The cell membrane shrank but the central vacuole remained full The cell membrane shrank away from the cell wall because the solution was hypertonic The cell became turgid
Biology
Biotechnology & its Applications
Multiple Choice O O The cell membrane did not change at all because of the protection of the cell wall The cells burst because the solution was hypotonic The cell membrane shrank but the central vacuole remained full The cell membrane shrank away from the cell wall because the solution was hypertonic The cell became turgid
Check All That Apply scalpel blades used gloves plastic petri dishes with cultures glass slide and cover glass disinfectant soaked paper towels used to clean a microbial spill
Biology
Biotechnology & its Applications
Check All That Apply scalpel blades used gloves plastic petri dishes with cultures glass slide and cover glass disinfectant soaked paper towels used to clean a microbial spill
2 1 point Match the factors for cheating with an appropriate exampl Cheating because social and academic lives have become too disorganized or stressful to manage Excuse making Performance anxiety Unfamiliarity with what constitutes cheating Peer pressure Self justification habits Inability to manage demands of student life False Blaming the reason for cheating on the course instructor being too hard Genuinely misunderstanding a course policy requiring individual work Cheating to compensate for poor test taking skills 1 point Cheating is justified if the professor has unfair or difficult questions on an exam True Cheating because social and a D Cheating is a broad term that encompasses many different ways that a student might attempt to gain academic credit in a way that is unfair deceitful or fraudulent Every semester at CSUB the Dean of Students receives reports from professors who have caught students attempting to cheat in one form or another Both professors and students are responsible for upholding the reputation of the university Cheating is often purposeful in nature but an unawareness of course or university policies and assignment requirements could also create confusion about what is and is not allowed Regardless of intention cheating can never be justified especially once you have learned the ways that it can negatively impact your college career and life outside the university Stay on the right path Know course policies on assignments collaboration exams Check the Syllabus If ethical dilemmas arise seek advice from trusted faculty or staff on campus on how you should deal with it Protect yourself It is your responsibility to clarify when collaboration is allowed and when it is not Different cultures and educational system can vary in how information is used and credited Reach out to your instructors early to avoid misunderstandings
Biology
Biotechnology & its Applications
2 1 point Match the factors for cheating with an appropriate exampl Cheating because social and academic lives have become too disorganized or stressful to manage Excuse making Performance anxiety Unfamiliarity with what constitutes cheating Peer pressure Self justification habits Inability to manage demands of student life False Blaming the reason for cheating on the course instructor being too hard Genuinely misunderstanding a course policy requiring individual work Cheating to compensate for poor test taking skills 1 point Cheating is justified if the professor has unfair or difficult questions on an exam True Cheating because social and a D Cheating is a broad term that encompasses many different ways that a student might attempt to gain academic credit in a way that is unfair deceitful or fraudulent Every semester at CSUB the Dean of Students receives reports from professors who have caught students attempting to cheat in one form or another Both professors and students are responsible for upholding the reputation of the university Cheating is often purposeful in nature but an unawareness of course or university policies and assignment requirements could also create confusion about what is and is not allowed Regardless of intention cheating can never be justified especially once you have learned the ways that it can negatively impact your college career and life outside the university Stay on the right path Know course policies on assignments collaboration exams Check the Syllabus If ethical dilemmas arise seek advice from trusted faculty or staff on campus on how you should deal with it Protect yourself It is your responsibility to clarify when collaboration is allowed and when it is not Different cultures and educational system can vary in how information is used and credited Reach out to your instructors early to avoid misunderstandings
8 1 point Which of the following is an example of cheating Return Sharing notes with a friend who missed class that day O Checking your smartwatch or phone for answers during a test or texting a friend for answers 000 0 1 point Which of the following are consequences of cheating Clarifying with your instructor whether or not you can use outside sources during an exam Asking a friend what citation format they ll be using for the final paper Submit Students who cheat will be unprepared for their future careers Its unlikely cheating will have many practical or social consequences besides lower grades Instructors are unable to accurately asses student performance or evaluate their mastery of knowledge in their field Cheating can become a habit that continues into other aspects of life and devalues hard work integrity and fairness Submit heating is a broad term that encompasses many different ways that a student might attempt to gain academic credit in a way that is unfair deceitful or fraudulent Every semester at CSUB the Dean of Students receives reports from professors who have caught students attempting to cheat in one form or another Both professors and students are responsible for upholding the reputation of the university Cheating is often purposeful in nature but an unawareness of course or university policies and assignment requirements could also create confusion about what is and is not allowed Regardless of intention cheating can never be justified especially once you have learned the ways that it can negatively impact your college career and life outside the university Stay on the right path Know course policies on assignments collaboration exams Check the Syllabus If ethical dilemmas arise seek advice from trusted faculty or staff on campus on how you should deal with it Protect yourself It is your responsibility to clarify when collaboration is allowed and when it is not Different cultures and educational systems can vary in how information is used and credited Reach out to your instructors early to avoid misunderstandings
Biology
Biotechnology & its Applications
8 1 point Which of the following is an example of cheating Return Sharing notes with a friend who missed class that day O Checking your smartwatch or phone for answers during a test or texting a friend for answers 000 0 1 point Which of the following are consequences of cheating Clarifying with your instructor whether or not you can use outside sources during an exam Asking a friend what citation format they ll be using for the final paper Submit Students who cheat will be unprepared for their future careers Its unlikely cheating will have many practical or social consequences besides lower grades Instructors are unable to accurately asses student performance or evaluate their mastery of knowledge in their field Cheating can become a habit that continues into other aspects of life and devalues hard work integrity and fairness Submit heating is a broad term that encompasses many different ways that a student might attempt to gain academic credit in a way that is unfair deceitful or fraudulent Every semester at CSUB the Dean of Students receives reports from professors who have caught students attempting to cheat in one form or another Both professors and students are responsible for upholding the reputation of the university Cheating is often purposeful in nature but an unawareness of course or university policies and assignment requirements could also create confusion about what is and is not allowed Regardless of intention cheating can never be justified especially once you have learned the ways that it can negatively impact your college career and life outside the university Stay on the right path Know course policies on assignments collaboration exams Check the Syllabus If ethical dilemmas arise seek advice from trusted faculty or staff on campus on how you should deal with it Protect yourself It is your responsibility to clarify when collaboration is allowed and when it is not Different cultures and educational systems can vary in how information is used and credited Reach out to your instructors early to avoid misunderstandings
Match the layer to the correct property crust mantle core lithosphere asthenosphere Choose highest rocky layer crust plus upper part of mantle solid middle rocky layer made of Fe iron partially molten layer of mantle Choose J Choose Choose
Biology
Biotechnology & its Applications
Match the layer to the correct property crust mantle core lithosphere asthenosphere Choose highest rocky layer crust plus upper part of mantle solid middle rocky layer made of Fe iron partially molten layer of mantle Choose J Choose Choose
During radioactive decay an isotope will lose mass and or energy and become a different element What changes in the nucleus makes it a different element O It loses gains one or more neutrons O It loses gains one or more protons
Biology
Biotechnology & its Applications
During radioactive decay an isotope will lose mass and or energy and become a different element What changes in the nucleus makes it a different element O It loses gains one or more neutrons O It loses gains one or more protons
Isotopes of an element differ by the number of protons they have O True False
Biology
Biotechnology & its Applications
Isotopes of an element differ by the number of protons they have O True False
Periods are shorter time increments than Eons O True O False
Biology
Biotechnology & its Applications
Periods are shorter time increments than Eons O True O False
Which of the following best scribes All dogs speak fluent Norwegian Mr Fluffington is a dog Therefore Mr Fluffington speaks fluent Norwegian Note Mr Fluffington is pictured below Invalid and unsound O Valid but unsound O Valid and sound
Biology
Biotechnology & its Applications
Which of the following best scribes All dogs speak fluent Norwegian Mr Fluffington is a dog Therefore Mr Fluffington speaks fluent Norwegian Note Mr Fluffington is pictured below Invalid and unsound O Valid but unsound O Valid and sound
Which resource allows the searcher to limit the results to scholarly journal articles only Google Scholar Google
Biology
Biotechnology & its Applications
Which resource allows the searcher to limit the results to scholarly journal articles only Google Scholar Google
If I make an error in my lab notebook I should draw a single line through the error and initial next to it erase it None of the other answer choices is correct just move to a new page and start over
Biology
Biotechnology & its Applications
If I make an error in my lab notebook I should draw a single line through the error and initial next to it erase it None of the other answer choices is correct just move to a new page and start over
An example of mimicry would be a butterfly that resembles a leaf Otwo species of frogs one poisonous that resemble one another in coloratio a minnow with spots that look like large eyes a carnivorous fish with a worm like tongue that lures prey
Biology
Biotechnology & its Applications
An example of mimicry would be a butterfly that resembles a leaf Otwo species of frogs one poisonous that resemble one another in coloratio a minnow with spots that look like large eyes a carnivorous fish with a worm like tongue that lures prey
Give an example of an advance in biotechnology that you have heard about in the news or read about in this chapter Explain the benefits of the application of biotechnology as well as possible negative effects
Biology
Biotechnology & its Applications
Give an example of an advance in biotechnology that you have heard about in the news or read about in this chapter Explain the benefits of the application of biotechnology as well as possible negative effects
True or False The Library Search Box option DATABASES provides a mechanism for finding library research databases alphabetically by title but NOT by subject O True False
Biology
Biotechnology & its Applications
True or False The Library Search Box option DATABASES provides a mechanism for finding library research databases alphabetically by title but NOT by subject O True False
You need resource recommendations for the field of genetics Which type of library guide should you try to find O Course O How To Current Topics
Biology
Biotechnology & its Applications
You need resource recommendations for the field of genetics Which type of library guide should you try to find O Course O How To Current Topics
A research or guiding question can be viewed through academic disciplinary lenses Which lens would best match this question How can society alleviate the issue of drug addiction O Sociology O Business O Criminal Justice Education
Biology
Biotechnology & its Applications
A research or guiding question can be viewed through academic disciplinary lenses Which lens would best match this question How can society alleviate the issue of drug addiction O Sociology O Business O Criminal Justice Education
The purpose of a good research or guiding question is what Select ALL that apply Involve a significant issue Help you focus and narrow your research Be relatable to others O Lead to analysis and conclusions
Biology
Biotechnology & its Applications
The purpose of a good research or guiding question is what Select ALL that apply Involve a significant issue Help you focus and narrow your research Be relatable to others O Lead to analysis and conclusions
socialism could only emerge in developed leaders communist capitalist economies Deng argued his policies were necessary as China still had feudal hang overs However such policies began a still on going debate about whether China has now become a capitalist country in all but name was based on the concept of economics in control Deng s approach rather than the Maoist idea of politics in control In other words had was more important than political dogma or economic pragmatism Practice versus Whatever In September 1982 the 12th Congress of the CCP approved Deng s economic plans as well as various personnel changes He was then in a position to introduce the second phase of his plans for industry DISCUSSION POINT Do you think that Deng s use of market mechanisms and allowing the existence of privately owned firms and enterprises automatically meant that China was likely to become a fully developed capitalist economy To discuss this in any depth you will need to do some research on key terminology and definitions such as capitalism socialism and market mechanisms Industry phase 2 1984 89 In October 1984 the Resolution on the Reform of the Economic System began Phase 2 This further reduced state control over enterprises though they still remained under public ownership Deng also made it clear that unprofitable enterprises would be closed down To make profits easier the state tax on enterprise profits was reduced from 55 to 33 in 1983 84 This emphasised that ownership and management were two separate aspects and that managers could be given some freedom in selecting ways to improve production In particular it allowed private groups to lease small and medium sized enterprises though the largest ones remained under direct government control The government also introduced a legal framework that protected private investment This as As the
Biology
Biotechnology & its Applications
socialism could only emerge in developed leaders communist capitalist economies Deng argued his policies were necessary as China still had feudal hang overs However such policies began a still on going debate about whether China has now become a capitalist country in all but name was based on the concept of economics in control Deng s approach rather than the Maoist idea of politics in control In other words had was more important than political dogma or economic pragmatism Practice versus Whatever In September 1982 the 12th Congress of the CCP approved Deng s economic plans as well as various personnel changes He was then in a position to introduce the second phase of his plans for industry DISCUSSION POINT Do you think that Deng s use of market mechanisms and allowing the existence of privately owned firms and enterprises automatically meant that China was likely to become a fully developed capitalist economy To discuss this in any depth you will need to do some research on key terminology and definitions such as capitalism socialism and market mechanisms Industry phase 2 1984 89 In October 1984 the Resolution on the Reform of the Economic System began Phase 2 This further reduced state control over enterprises though they still remained under public ownership Deng also made it clear that unprofitable enterprises would be closed down To make profits easier the state tax on enterprise profits was reduced from 55 to 33 in 1983 84 This emphasised that ownership and management were two separate aspects and that managers could be given some freedom in selecting ways to improve production In particular it allowed private groups to lease small and medium sized enterprises though the largest ones remained under direct government control The government also introduced a legal framework that protected private investment This as As the
How long would the peptide be that is translated from this mRNA sequence 5 AUGGGCUACCGA 3 O0 amino acids O 3 amino acids 2 amino acids 4 amino acids
Biology
Biotechnology & its Applications
How long would the peptide be that is translated from this mRNA sequence 5 AUGGGCUACCGA 3 O0 amino acids O 3 amino acids 2 amino acids 4 amino acids
No GFP 100 25 10 4 2 Counts Counts Counts Counts 50 100 0 10 Counts 50 0 10 100 50 100 0 10 10 50 10 100 10 0 10 10 50 0 1 10 GFP 87 77 10 GFP 10 10 GFP 82 83 10 104 10 104 81 21 104 10 10 GFP 73 13 104 0 10 10 10 10 104 GFP Figure S11 Transfection Efficiency of the PLAT E Cells and pMX System We transfected the GFP expressing retroviral vector and the control vector at ratios of 1 0 1 3 1 9 and 1 23 into MEFs with a constant amount of total DNA Forty eight hours later cells were photographed under a fluorescence microscope and analyzed by flow cytometry Bars indicate 200 m The analysis showed that 88 83 81 and 73 of transfected cells expressed GFP the mean GFP fluorescence being weaker with decreased concentration of the GFP expressing vector This indicates that 47 0 83 12 0 81 and 0 05 0 7324 of cells transfected with the four 10 or 24 factors expressed all the factors respectively In a 100 mm dish we plated 8 x 105 cells of which 4 x 10 cells should express all the four selected
Biology
Biotechnology & its Applications
No GFP 100 25 10 4 2 Counts Counts Counts Counts 50 100 0 10 Counts 50 0 10 100 50 100 0 10 10 50 10 100 10 0 10 10 50 0 1 10 GFP 87 77 10 GFP 10 10 GFP 82 83 10 104 10 104 81 21 104 10 10 GFP 73 13 104 0 10 10 10 10 104 GFP Figure S11 Transfection Efficiency of the PLAT E Cells and pMX System We transfected the GFP expressing retroviral vector and the control vector at ratios of 1 0 1 3 1 9 and 1 23 into MEFs with a constant amount of total DNA Forty eight hours later cells were photographed under a fluorescence microscope and analyzed by flow cytometry Bars indicate 200 m The analysis showed that 88 83 81 and 73 of transfected cells expressed GFP the mean GFP fluorescence being weaker with decreased concentration of the GFP expressing vector This indicates that 47 0 83 12 0 81 and 0 05 0 7324 of cells transfected with the four 10 or 24 factors expressed all the factors respectively In a 100 mm dish we plated 8 x 105 cells of which 4 x 10 cells should express all the four selected
Unit Chemical Writing Formula Equations WS 1 Directions Convert the following word equations into formula equations by changing element and compound names into chemical formulas 1 sodium oxygen sodium oxide 2 copper silver nitrate copper II nitrate silver 3 aluminum chlorate barium sulfate aluminum sulfate barium chlorate 4 bromine calcium iodide calcium bromide iodine 5 zinc sulfide magnesium arsenate zinc arsenate magnesium sulfide 6 cobalt lead II nitrite cobalt III nitrite lead muptorpotassium hydroxide hydrogen qmsx3
Biology
Biotechnology & its Applications
Unit Chemical Writing Formula Equations WS 1 Directions Convert the following word equations into formula equations by changing element and compound names into chemical formulas 1 sodium oxygen sodium oxide 2 copper silver nitrate copper II nitrate silver 3 aluminum chlorate barium sulfate aluminum sulfate barium chlorate 4 bromine calcium iodide calcium bromide iodine 5 zinc sulfide magnesium arsenate zinc arsenate magnesium sulfide 6 cobalt lead II nitrite cobalt III nitrite lead muptorpotassium hydroxide hydrogen qmsx3
4 Ionic compounds In the chart below write the ionic formula if it gives you the ionic compound name Write the ionic compound name if it gives you the formula lonic Compound Name Iron III chloride Ammonium phosphate Tin IV sulfate Potassium perchlorate Aluminum hydroxide Sodium chloride Calcium fluoride Potassium bromide Magnesium carbonate Formula lonic Compound Name Formula NaF Bel2 Al2O3 FeF3 PbO CaSO3 Ba3 PO4 2 NH4 2CO2 Cul2
Biology
Biotechnology & its Applications
4 Ionic compounds In the chart below write the ionic formula if it gives you the ionic compound name Write the ionic compound name if it gives you the formula lonic Compound Name Iron III chloride Ammonium phosphate Tin IV sulfate Potassium perchlorate Aluminum hydroxide Sodium chloride Calcium fluoride Potassium bromide Magnesium carbonate Formula lonic Compound Name Formula NaF Bel2 Al2O3 FeF3 PbO CaSO3 Ba3 PO4 2 NH4 2CO2 Cul2
Gene therapy detailed information about the structure organization and function of the complete set of human genes the process of determining the precise order of nucleotides within a DNA molecule aims to treat a disease by replacing mutated or defective gene with a functional allele process of cultivating cells and tissues outside the body of an organism in vitro in an artificial
Biology
Biotechnology & its Applications
Gene therapy detailed information about the structure organization and function of the complete set of human genes the process of determining the precise order of nucleotides within a DNA molecule aims to treat a disease by replacing mutated or defective gene with a functional allele process of cultivating cells and tissues outside the body of an organism in vitro in an artificial
Sexual Isolati 2 5 15 1 0 5 A B Same Hosts ecologically similar Based on the graph which of the following predictions can be made regarding the ecological speciation of both types of he An ecologically similar pair of population exhibits greater reproductive isolation increased level of gene flow population C Different Hosts ecologically diverse Type of Population Pair An ecologically diverse pair of population exhibits a lower reproductive isolation decreased level of gene flo population An ecologically similar pair of population exhibits a lower reproductive isolation decreased level of gene flow population
Biology
Biotechnology & its Applications
Sexual Isolati 2 5 15 1 0 5 A B Same Hosts ecologically similar Based on the graph which of the following predictions can be made regarding the ecological speciation of both types of he An ecologically similar pair of population exhibits greater reproductive isolation increased level of gene flow population C Different Hosts ecologically diverse Type of Population Pair An ecologically diverse pair of population exhibits a lower reproductive isolation decreased level of gene flo population An ecologically similar pair of population exhibits a lower reproductive isolation decreased level of gene flow population
Draw a diagram that illustrates the experimental design described in this excerpt Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells To identify candidate reprogramming factors we compiled a list of genes with enriched expression in human ES cells relative to that of other cell types and prioritized the list based on known involvement in the establishment or maintenance of pluripotency We then cloned these genes into a lentiviral vector to screen for combinations of genes that could reprogram the differentiated derivatives of an OCT4 knock in human ES cell line generated through homologous recombination In this cell line the expression of neomycin phosphotransferase which makes cells resistant to geneticin is driven by an endogenous OCT4 promoter a gene that is highly expressed in pluripotent cells but not in differentiated cells Thus reprogramming events reactivating the OCT4 promoter
Biology
Biotechnology & its Applications
Draw a diagram that illustrates the experimental design described in this excerpt Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells To identify candidate reprogramming factors we compiled a list of genes with enriched expression in human ES cells relative to that of other cell types and prioritized the list based on known involvement in the establishment or maintenance of pluripotency We then cloned these genes into a lentiviral vector to screen for combinations of genes that could reprogram the differentiated derivatives of an OCT4 knock in human ES cell line generated through homologous recombination In this cell line the expression of neomycin phosphotransferase which makes cells resistant to geneticin is driven by an endogenous OCT4 promoter a gene that is highly expressed in pluripotent cells but not in differentiated cells Thus reprogramming events reactivating the OCT4 promoter
Humans are direct descendants of chimpanzees This is one of the problems with studying evolution none of the other modern primates have their own fossil records to trait their evolution Check your answer in section 5 O True False
Biology
Biotechnology & its Applications
Humans are direct descendants of chimpanzees This is one of the problems with studying evolution none of the other modern primates have their own fossil records to trait their evolution Check your answer in section 5 O True False